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X-ray diffraction
1.75Å resolution

Bacillus cereus Alkylpurine DNA Glycosylase AlkD Bound to DNA Containing an Abasic Site (across from T)

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-116458 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA alkylation repair protein Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 26.97 KDa
Source organism: Bacillus cereus ATCC 10987
Expression system: Escherichia coli
UniProt:
  • Canonical: Q816E8 (Residues: 1-225; Coverage: 95%)
Gene name: BC_4913
Sequence domains: DNA alkylation repair enzyme
Structure domains: Leucine-rich Repeat Variant
DNA (5'-D(*TP*GP*GP*GP*(3DR)P*GP*GP*CP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 2.98 KDa
Source organism: Bacillus cereus ATCC 10987
Expression system: Not provided
DNA (5'-D(*AP*AP*AP*GP*CP*CP*TP*CP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 2.97 KDa
Source organism: Bacillus cereus ATCC 10987
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 52.333Å b: 57.445Å c: 53.655Å
α: 90° β: 101.88° γ: 90°
R-values:
R R work R free
0.203 0.184 0.225
Expression systems:
  • Escherichia coli
  • Not provided