› 3juo

X-ray diffraction
2.2Å resolution

Crystal Structure of PhzA/B from Burkholderia cepacia R18194 in complex with (R)-5-bromo-2-(piperidin-3-ylamino)benzoic acid

Released:
DOI: 10.2210/pdb3juo/pdb
PDB 3juo coloured by chain and viewed from the front.
PDB 3juo coloured by chain and viewed from the side.
PDB 3juo coloured by chain and viewed from the top.
Source organism: Burkholderia lata
Primary publication:
The active site of an enzyme can host both enantiomers of a racemic ligand simultaneously.
Mentel M, Blankenfeldt W, Breinbauer R
Angew. Chem. Int. Ed. Engl. 48 9084-7 (2009)
PMID: 19876985

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenazine biosynthesis protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 185 amino acids
Theoretical weight: 21.53 KDa
Source organism: Burkholderia lata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q396C9 (Residues: 1-165; Coverage: 100%)
Gene name: Bcep18194_B1568
Sequence domains: Phenazine biosynthesis protein A/B
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand AJD 2 x AJD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 64.6Å b: 64.6Å c: 160.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.223
Expression system: Escherichia coli

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Citations

3 review citations

Recent developments in the isolation, biological function, biosynthesis, and synthesis of phenazine natural products.
Guttenberger et al. (2017)
2 more