3jtg

X-ray diffraction
2.2Å resolution

Crystal structure of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-beta receptor promoter DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ETS-related transcription factor Elf-3 Chain: A
Molecule details ›
Chain: A
Length: 103 amino acids
Theoretical weight: 12.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3UPW2 (Residues: 289-391; Coverage: 26%)
Gene names: Elf3, Ert, Esx, Jen
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*GP*AP*GP*GP*AP*GP*TP*TP*TP*CP*CP*TP*GP*TP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.94 KDa
Source organism: Mus musculus
Expression system: Not provided
DNA (5'-D(*CP*AP*AP*AP*CP*AP*GP*GP*AP*AP*AP*CP*TP*CP*CP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 42.66Å b: 52.005Å c: 99.783Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.263
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided