3jbr

Electron Microscopy
4.2Å resolution

Cryo-EM structure of the rabbit voltage-gated calcium channel Cav1.1 complex at 4.2 angstrom

Released:
Primary publication:
Structure of the voltage-gated calcium channel Cav1.1 complex.
Science 350 aad2395 (2015)
PMID: 26680202
Related structures: EMD-6475

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Voltage-dependent L-type calcium channel subunit alpha-1S Chain: A
Molecule details ›
Chain: A
Length: 1873 amino acids
Theoretical weight: 208.66 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P07293 (Residues: 1-1873; Coverage: 100%)
Gene names: CACH1, CACNA1S, CACNL1A3
Sequence domains:
Voltage-dependent L-type calcium channel subunit beta-2 Chain: B
Molecule details ›
Chain: B
Length: 356 amino acids
Theoretical weight: 40.3 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VGC3 (Residues: 68-196, 254-476; Coverage: 54%)
  • Best match: Q8VGC3-4 (Residues: 24-152, 210-432)
Gene names: Cacnb2, Cacnlb2
Sequence domains:
Voltage-dependent calcium channel gamma-1 subunit Chain: E
Molecule details ›
Chain: E
Length: 222 amino acids
Theoretical weight: 25.08 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P19518 (Residues: 1-222; Coverage: 100%)
Gene names: CACNG1, CACNLG
Sequence domains: PMP-22/EMP/MP20/Claudin tight junction
Voltage-dependent calcium channel subunit alpha-2/delta-1 Chain: F
Molecule details ›
Chain: F
Length: 1106 amino acids
Theoretical weight: 116.78 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P13806 (Residues: 1-1106; Coverage: 100%)
Gene names: CACNA2D1, CACNL2A, CCHL2A
Sequence domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-6475
Expression system: Escherichia coli