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Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)

Electron Microscopy
7.6Å resolution
PDB entry 3j96 coloured by chain, front view.
PDB entry 3j96 coloured by chain, side view.
PDB entry 3j96 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero tridecamer (preferred)
Entry contents: 5 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tridecamer
Accessible surface area: 257198.89 Å2
Buried surface area: 55003.58 Å2
Dissociation area: 8,962.4 Å2
Dissociation energy (ΔGdiss): -8.26 kcal/mol
Dissociation entropy (TΔSdiss): 75.16 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148439

Macromolecules

Vesicle-fusing ATPase
Chains: A, B, C, D, E, F
Length: 747 amino acids
Theoretical weight: 82.91 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18708 (Residues: 1-744; Coverage: 100%)
Gene name: NSF
Pfam:
InterPro:
Vesicle-fusing ATPase in PDB entry 3j96, assembly 1, front view.
Vesicle-fusing ATPase in PDB entry 3j96, assembly 1, side view.
Vesicle-fusing ATPase in PDB entry 3j96, assembly 1, top view.
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Alpha-soluble NSF attachment protein
Chains: G, H, I, J
Length: 297 amino acids
Theoretical weight: 33.38 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54921 (Residues: 1-295; Coverage: 100%)
Gene names: Napa, Snap, Snapa
InterPro:
Alpha-soluble NSF attachment protein in PDB entry 3j96, assembly 1, front view.
Alpha-soluble NSF attachment protein in PDB entry 3j96, assembly 1, side view.
Alpha-soluble NSF attachment protein in PDB entry 3j96, assembly 1, top view.
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Vesicle-associated membrane protein 2
Chain: K
Length: 63 amino acids
Theoretical weight: 7.23 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63045 (Residues: 28-89; Coverage: 53%)
Gene names: Syb2, Vamp2
Pfam: Synaptobrevin
InterPro:
Vesicle-associated membrane protein 2 in PDB entry 3j96, assembly 1, front view.
Vesicle-associated membrane protein 2 in PDB entry 3j96, assembly 1, side view.
Vesicle-associated membrane protein 2 in PDB entry 3j96, assembly 1, top view.
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Syntaxin-1A
Chain: L
Length: 67 amino acids
Theoretical weight: 7.84 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 191-256; Coverage: 23%)
Gene names: Sap, Stx1a
Pfam: SNARE domain
InterPro:
Syntaxin-1A in PDB entry 3j96, assembly 1, front view.
Syntaxin-1A in PDB entry 3j96, assembly 1, side view.
Syntaxin-1A in PDB entry 3j96, assembly 1, top view.
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Synaptosomal-associated protein 25
Chain: M
Length: 188 amino acids
Theoretical weight: 21.26 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 17-204; Coverage: 91%)
Gene names: Snap, Snap25
Pfam: SNAP-25 family
InterPro:
Synaptosomal-associated protein 25 in PDB entry 3j96, assembly 1, front view.
Synaptosomal-associated protein 25 in PDB entry 3j96, assembly 1, side view.
Synaptosomal-associated protein 25 in PDB entry 3j96, assembly 1, top view.
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