3j79

Electron Microscopy
3.2Å resolution

Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine, large subunit

Released:

Function and Biology Details

Reactions catalysed:
Hydrolysis of proteins in presence of ATP.
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate
D-glucose + O(2) = 2-dehydro-D-glucose + H(2)O(2)
Geranyl diphosphate = alpha-terpinene + diphosphate
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
H(2) + 2 oxidized ferredoxin = 2 reduced ferredoxin + 2 H(+)
L-serine + L-homocysteine = L-cystathionine + H(2)O
Propane-1,2-diol = propanal + H(2)O
Trypanothione + NADP(+) = trypanothione disulfide + NADPH
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
NTP + H(2)O = NDP + phosphate
Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
(+)-muconolactone = 5-oxo-4,5-dihydrofuran-2-acetate
(1a) ATP + [RNA ligase]-L-lysine = [RNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
D-glucose 6-phosphate = D-fructose 6-phosphate
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
D-ribose 5-phosphate = D-ribulose 5-phosphate
Cutin + H(2)O = cutin monomers
Geranyl diphosphate = gamma-terpinene + diphosphate
S-(hydroxymethyl)glutathione + NAD(P)(+) = S-formylglutathione + NAD(P)H
2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde
Methylamine + H(2)O + 2 oxidized [amicyanin] = formaldehyde + NH(3) + 2 reduced [amicyanin]
ATP + thymidine = ADP + thymidine 5'-phosphate
Geranylgeranyl diphosphate = (+)-copalyl diphosphate
A glutathione-S-conjugate + H(2)O = an (L-cysteinylglycine)-S-conjugate + L-glutamate
NH(3) + 2 H(2)O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H(+)
L-carnitine + NAD(P)H + O(2) = (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD(P)(+) + H(2)O
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH
L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + a UDP-3-O-((3R)-hydroxyacyl)-alpha-D-glucosamine = a UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + a holo-[acyl-carrier-protein]
(+)-trans-carveol + NAD(+) = (+)-(S)-carvone + NADH
2 nitric oxide + 2 O(2) + NAD(P)H = 2 nitrate + NAD(P)(+) + H(+)
Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H(2)O
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
ATP + (L-Asp(4-L-Arg))(n) + L-Asp = ADP + phosphate + (L-Asp(4-L-Arg))(n)-L-Asp
4-fumarylacetoacetate + H(2)O = acetoacetate + fumarate
ATP + a protein = ADP + a phosphoprotein
A glycerophosphodiester + H(2)O = an alcohol + sn-glycerol 3-phosphate
A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol
ATP-dependent breakage, passage and rejoining of double-stranded DNA
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier-protein] + a UDP-3-O-(3-hydroxyacylyl)-N-acetyl-alpha-D-glucosamine
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
S-adenosyl-L-methionine = S-adenosyl 3-(methylthio)propylamine + CO(2)
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
(1a) L-tyrosine + 1/2 O(2) = L-dopa
L-asparagine + H(2)O = L-aspartate + NH(3)
Urea + H(2)O = CO(2) + 2 NH(3)
Triphosphate + [microsomal-membrane protein] = diphosphate + [microsomal-membrane protein] phosphate
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA
(1a) L-glutamine + H(2)O = L-glutamate + NH(4)(+)
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate
4 ferrocytochrome c + O(2) + 4 H(+) = 4 ferricytochrome c + 2 H(2)O
RX + glutathione = HX + R-S-glutathione
(1a) 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] = 2 [corrinoid adenosyltransferase]-cob(II)alamin
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate
Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
(1a) L-methionine = methanethiol + 2-aminobut-2-enoate
2-phospho-D-glycerate = 3-phospho-D-glycerate
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
2 H(2)O(2) = O(2) + 2 H(2)O
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate
Prenyl phosphate + FMNH(2) = prenylated FMNH(2) + phosphate
(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Methyl-CoM + CoB = CoM-S-S-CoB + methane
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
A purine nucleoside + H(2)O = D-ribose + a purine base
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
L-histidine = urocanate + NH(3)
Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2) 
An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH
L-ornithine = L-proline + NH(3)
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
H(2)CO(3) = CO(2) + H(2)O
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
NAD(H) + H(2)O = AMP + NMN(H)
dUTP + H(2)O = dUMP + diphosphate
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
(1a) (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H(2)O
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose
2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + phenylacetate + CoA = AMP + diphosphate + phenylacetyl-CoA
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Carbamoyl phosphate + N(2)-succinyl-L-ornithine = phosphate + N-succinyl-L-citrulline
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
A beta-lactam + H(2)O = a substituted beta-amino acid
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
5,6,7,8-tetrahydrobiopterin + 2 NADP(+) = biopterin + 2 NADPH
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H(2)O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine
2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
NADPH + NAD(+) + H(+)(Side 1) = NADP(+) + H(+)(Side 2) + NADH
2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
N,N-dimethylformamide + H(2)O = dimethylamine + formate
Ethylnitronate + O(2) = acetaldehyde + nitrite + other products
Cyclomaltodextrin + H(2)O = linear maltodextrin
UDP-glucose + 2 NAD(+) + H(2)O = UDP-glucuronate + 2 NADH
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine
Cytosine + H(2)O = uracil + NH(3)
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Succinate + a quinone = fumarate + a quinol
IMP + H(2)O = inosine + phosphate
Salicylate + NADH + O(2) = catechol + NAD(+) + H(2)O + CO(2)
ATP + H(2)O = ADP + phosphate
L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
4 sulfur + 4 H(2)O + O(2) = 2 H(2)S + 2 HSO(3)(-) + 2 H(+)
D-arabinose 5-phosphate = D-ribulose 5-phosphate
4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate
Release of N-terminal proline from a peptide.
A phosphate monoester + H(2)O = an alcohol + phosphate
Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
Diphosphate + H(2)O = 2 phosphate
L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O
An acylphosphate + H(2)O = a carboxylate + phosphate
An aliphatic amide = a nitrile + H(2)O
(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate
(R)-S-lactoylglutathione = glutathione + methylglyoxal
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m)
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain
4 porphobilinogen + H(2)O = hydroxymethylbilane + 4 NH(3)
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
S-methyl-5'-thioadenosine + H(2)O = 5-(methylsulfanyl)-D-ribose + adenine
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Maltose = alpha,alpha-trehalose
ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
3-deoxy-D-manno-octulosonate 8-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate + phosphate
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
(R)-2-haloacid + H(2)O = (S)-2-hydroxyacid + halide
A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]
Acetyl-CoA + L-2,4-diaminobutanoate = CoA + N(4)-acetyl-L-2,4-diaminobutanoate
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Cleavage of peptide bonds with very broad specificity.
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Glycerone phosphate = methylglyoxal + phosphate
Propane-1,3-diol + NAD(+) = 3-hydroxypropanal + NADH
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
(1a) Man(9)GlcNAc(2)-[protein] + H(2)O = Man(8)GlcNAc(2)-[protein] (isomer 8A(1,2,3)B(1,2)) + beta-D-mannopyranose
Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Formamide + H(2)O = formate + NH(3)
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
4-(2-carboxyphenyl)-4-oxobutanoyl-CoA = 1,4-dihydroxy-2-naphthoyl-CoA + H(2)O
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate
ATP + (L-Asp(4-L-Arg))(n)-L-Asp + L-Arg = ADP + phosphate + (L-Asp(4-L-Arg))(n+1)
S-adenosyl-L-methionine + a [sulfatase]-L-cysteine + H(2)O = a [sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine + hydrogen sulfide
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
N-carbamoylputrescine + H(2)O = putrescine + CO(2) + NH(3)
A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
ATP + D-ribose = ADP + D-ribose 5-phosphate
Chloride + O(2) = chlorite
(1a) 2 Fe(2+) + O(2) + 4 H(2)O = 2 (FeO(OH)) + 4 H(+) + H(2)O(2)
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
A 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranoside + H(2)O = 6-O-(beta-D-xylopyranosyl)-beta-D-glucopyranose + an alcohol
D-glyceraldehyde 3-phosphate = glycerone phosphate
L-aspartate = D-aspartate
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine
O-phospho-L-homoserine + H(2)O = L-threonine + phosphate
CO + H(2)O + 2 oxidized ferredoxin = CO(2) + 2 reduced ferredoxin + 2 H(+)
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
ATP + AMP = 2 ADP
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(36) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(36)
5-hydroxyisourate + H(2)O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate = (15Z)-12-oxophyto-10,15-dienoate
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Propanoyl-CoA + phosphate = CoA + propanoyl phosphate
(S)-acetoin + NAD(+) = diacetyl + NADH
GTP + H(2)O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
An aryl sulfate + H(2)O = a phenol + sulfate
6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate
(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)-2'-phosphate
FAD + [protein]-L-threonine = [protein]-FMN-L-threonine + AMP
Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate = oxaloacetate + pyruvate
H(2) + 2 ferricytochrome c3 = 2 H(+) + 2 ferrocytochrome c3
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H
UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
L-arginine + 2-oxoglutarate + O(2) = (3S)-3-hydroxy-L-arginine + succinate + CO(2)
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
ATP + glycerol = ADP + sn-glycerol 3-phosphate
A pyrimidine nucleoside + H(2)O = D-ribose + a pyrimidine base
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Glycolate + NADP(+) = glyoxylate + NADPH
(2R,3S)-catechin + NADP(+) + H(2)O = 2,3-trans-3,4-cis-leucocyanidin + NADPH
D-glycerate + NAD(P)(+) = hydroxypyruvate + NAD(P)H
Catechol + O(2) = 2-hydroxymuconate-6-semialdehyde
dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose
L-fucopyranose = L-fuculose
Oxalyl-CoA = formyl-CoA + CO(2)
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Benzoylformate = benzaldehyde + CO(2)
Citrate = acetate + oxaloacetate
Aceneneuramate = N-acetyl-D-mannosamine + pyruvate
ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Peptidylproline (omega=180) = peptidylproline (omega=0)
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
L-histidinol + H(2)O + 2 NAD(+) = L-histidine + 2 NADH
(S)-lactate + NAD(+) = pyruvate + NADH
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec)
ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H(2)O
Tetrahydropteroyl-(gamma-glutamyl)(n) + (n-1) H(2)O = 5,6,7,8-tetrahydrofolate + (n-1) L-glutamate
Oligoglycosylglucosyl-(1<->1)-ceramide + H(2)O = ceramide + oligoglycosylglucose
Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
Hydrolysis of Xaa-|-Pro dipeptides. Also acts on aminoacyl-hydroxyproline analogs. No action on Pro-|-Pro.
L-glutamine + H(2)O = L-glutamate + NH(3)
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
ADP-alpha-D-glucose + D-glucose 6-phosphate = ADP + alpha,alpha-trehalose 6-phosphate
(1a) 2 geranylgeranyl diphosphate = diphosphate + prephytoene diphosphate
L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH
Nitrite + acceptor = nitrate + reduced acceptor
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 
ATP + adenosine = ADP + AMP
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
ATP + 3-methylbut-3-en-1-yl phosphate = ADP + 3-methylbut-3-en-1-yl diphosphate
L-arginine + H(2)O = L-ornithine + urea
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
S-formylglutathione + H(2)O = glutathione + formate
S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA 
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate
Creatinine + H(2)O = creatine
Oxalate + O(2) + 2 H(+) = 2 CO(2) + H(2)O(2)
4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
Pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'-phosphate + NH(3) + H(2)O(2)
Protoheme + 3 [reduced NADPH--hemoprotein reductase] + 3 O(2) = biliverdin + Fe(2+) + CO + 3 [oxidized NADPH--hemoprotein reductase] + 3 H(2)O
A C(21)-steroid + [reduced NADPH--hemoprotein reductase] + O(2) = a 17-alpha-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H(2)O
D-ribulose 5-phosphate = D-xylulose 5-phosphate
Phosphatidylinositol 3,4,5-trisphosphate + H(2)O = phosphatidylinositol 4,5-bisphosphate + phosphate
Acyl-CoA + H(2)O = CoA + a carboxylate
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + NADPH
Prephenate = phenylpyruvate + H(2)O + CO(2)
(4Z)-2-oxohept-4-enedioate + H(2)O = (4S)-4-hydroxy-2-oxoheptanedioate
Cellobiose + phosphate = alpha-D-glucose 1-phosphate + D-glucose
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH
S-adenosyl-L-homocysteine + H(2)O = L-homocysteine + adenosine
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein
Beta-D-ribopyranose = beta-D-ribofuranose
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
D-fructose 6-phosphate + phosphate = acetyl phosphate + D-erythrose 4-phosphate + H(2)O
7,8-dihydroneopterin 3'-triphosphate + H(2)O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 
D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate
4-hydroxy-2-oxohexanoate = (2Z,4Z)-2-hydroxyhexa-2,4-dienoate + H(2)O
ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein]
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
17-alpha-hydroxypregnenolone + [reduced NADPH--hemoprotein reductase] + O(2) = 3-beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH--hemoprotein reductase] + H(2)O
ATP + sulfate = diphosphate + adenylyl sulfate
(1a) 2 farnesyl diphosphate = diphosphate + presqualene diphosphate
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol
Acetyl-CoA + glycine = CoA + 2-amino-3-oxobutanoate
ATP + protoporphyrin IX + Mg(2+) + H(2)O = ADP + phosphate + Mg-protoporphyrin IX + 2 H(+)
2'-deoxycytidine + H(2)O = 2'-deoxyuridine + NH(3)
ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)
Quercetin + O(2) = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H(+)
(S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-dienoate + H(2)O
A carboxylic ester + H(2)O = an alcohol + a carboxylate
ATP + pyruvate = ADP + phosphoenolpyruvate
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate
2 reduced [2Fe-2S] ferredoxin + NAD(+) + H(+) = 2 oxidized [2Fe-2S] ferredoxin + NADH
Succinyl-CoA + acetyl-CoA = CoA + 3-oxoadipyl-CoA
Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
(3E)-2-oxohex-3-enedioate = 2-oxopent-4-enoate + CO(2)
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO(3)(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
An alcohol + NADP(+) = an aldehyde + NADPH
NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
Endonucleolytic cleavage to 5'-phosphomonoester.
2 5-aminolevulinate = porphobilinogen + 2 H(2)O
L-glutamate = D-glutamate
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
ATP + RNA(n) = diphosphate + RNA(n+1)
((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate
An L-amino acid = a D-amino acid
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
A phosphatidylcholine + H(2)O = choline + a phosphatidate
(2E,6E)-farnesyl diphosphate = amorpha-4,11-diene + diphosphate
N(6)-prenyladenine + acceptor + H(2)O = adenine + 3-methylbut-2-enal + reduced acceptor
ATP + kanamycin = ADP + kanamycin 3'-phosphate
L-phenylalanine + tetrahydrobiopterin + O(2) = L-tyrosine + 4a-hydroxytetrahydrobiopterin
Beta-nicotinate D-ribonucleotide + diphosphate + CO(2) = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate
3-dehydroquinate = 3-dehydroshikimate + H(2)O
ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine
ATP + deamido-NAD(+) + L-glutamine + H(2)O = AMP + diphosphate + NAD(+) + L-glutamate
A chalcone = a flavanone
Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
(R)-3-hydroxyacyl-CoA + NADP(+) = 3-oxoacyl-CoA + NADPH
A primary alcohol + 2 cytochrome c(L) = an aldehyde + 2 reduced cytochrome c(L) + 2 H(+)
ATP + pyridoxal = ADP + pyridoxal 5'-phosphate
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate
Formate + NAD(+) = CO(2) + NADH
ATP + D-glucose = ADP + D-glucose 6-phosphate
NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+)
N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate
Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin
S-adenosyl-L-methionine + 3-((1-carboxyvinyl)oxy)benzoate + H(2)O = 6-amino-6-deoxyfutalosine + L-methionine + HCO(3)(-)
(1a) cyanate + HCO(3)(-) + H(+) = carbamate + CO(2)
Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
ATP + propanoyl-CoA + HCO(3)(-) = ADP + phosphate + (S)-methylmalonyl-CoA
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)
Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
L-cystathionine + H(2)O = L-homocysteine + NH(3) + pyruvate
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
(S)-dihydroorotate + fumarate = orotate + succinate
NAD(+) + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H(2)O
Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).
ATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO(2)
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl)
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H(2)O
H(2) + NAD(+) = H(+) + NADH
H(2) + A = AH(2)
4-coumaroyl-CoA + shikimate = CoA + 4-coumaroylshikimate
(1a) RNA = an (RNA fragment)-3'-nucleoside-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+)
(1a) dTDP-beta-L-evernosamine + NADPH + O(2) = dTDP-N-hydroxy-beta-L-evernosamine + NADP(+) + H(2)O
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate
An aryl dialkyl phosphate + H(2)O = dialkyl phosphate + an aryl alcohol
2 putrescine = sym-homospermidine + NH(3)
O-acetyl-L-homoserine + methanethiol = L-methionine + acetate
Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose
Oxaloacetate = pyruvate + CO(2)
S-adenosyl-L-methionine + guanine(1405) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(1405) in 16S rRNA 
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H(2)O
Carbamoyl phosphate + N(2)-acetyl-L-ornithine = phosphate + N-acetyl-L-citrulline
S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.
(+)-muconolactone = cis,cis-muconate
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Similar to that of papain.
Preferential cleavage of -Tyr(16)-|-Leu- and -Phe(25)-|-Tyr-bonds of oxidized insulin B chain. Also acts on other substrates of less than 7 kDa such as insulin and glucagon.
L-histidinol phosphate + H(2)O = L-histidinol + phosphate
Hydrazine + 4 ferricytochrome c = N(2) + 4 ferrocytochrome c
(1a) S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = S-methyl-5'-thioadenosine + N(4)-aminopropylspermidine
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
D-sorbitol 6-phosphate + NAD(+) = D-fructose 6-phosphate + NADH
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Prenyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate
ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N(6)-L-lysine
Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.
Phosphonoacetaldehyde + H(2)O = acetaldehyde + phosphate
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
A 2-oxo acid = an aldehyde + CO(2)
L-threonine + NAD(+) = L-2-amino-3-oxobutanoate + NADH
(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate
Malonyl-CoA = acetyl-CoA + CO(2)
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
(R)-mandelonitrile = cyanide + benzaldehyde
Vinylacetyl-CoA = (E)-but-2-enoyl-CoA
2 pyruvate = 2-acetolactate + CO(2)
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine
(S)-dihydroorotate + a quinone = orotate + a quinol
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites
4-trimethylammoniobutanoate + 2-oxoglutarate + O(2) = 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO(2) 
ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
(S)(+)-allantoin = (R)(-)-allantoin
(S)-3-hydroxybutanoyl-CoA + NADP(+) = 3-acetoacetyl-CoA + NADPH
L-allo-threonine = glycine + acetaldehyde
NADH + ROOH + H(+) = NAD(+) + H(2)O + ROH
A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
An alpha-amino acid ester + H(2)O = an alpha-amino acid + an alcohol
Choline = trimethylamine + acetaldehyde
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine
2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH
ADP-D-ribose 1''-phosphate + H(2)O = ADP-D-ribose + phosphate
Glycerol + NAD(+) = glycerone + NADH
A primary alcohol + NAD(+) = an aldehyde + NADH
L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H
N-acyl-D-glutamate + H(2)O = a carboxylate + D-glutamate
A 2'-deoxyribonucleoside 5'-monophosphate + H(2)O = a 2'-deoxyribonucleoside + phosphate
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH(3)
L-lysyl-tRNA(Lys) + phosphatidylglycerol = tRNA(Lys) + 3-O-L-lysyl-1-O-phosphatidylglycerol
(1a) a [histone H3]-N(6),N(6)-dimethyl-L-lysine(9) + 2-oxoglutarate + O(2) = a [histone H3]-N(6)-methyl-L-lysine(9) + succinate + formaldehyde + CO(2)
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(In) = 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na(+)(Out)
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Xyloglucan + H(2)O = xyloglucan oligosaccharides
Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.
1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H(2)O + phosphate
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
A 4-O-methyl-D-glucopyranuronate ester + H(2)O = 4-O-methyl-D-glucuronic acid + an alcohol
ATP + dTMP = ADP + dTDP
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Acetaldehyde + CoA + NAD(+) = acetyl-CoA + NADH
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
An alkanesulfonate (R-CH(2)-SO(3)H) + FMNH(2) + O(2) = an aldehyde (R-CHO) + FMN + sulfite + H(2)O
3-oxoadipate enol-lactone + H(2)O = 3-oxoadipate
A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
1-haloalkane + H(2)O = a primary alcohol + halide
(S)-malate + NAD(+) = oxaloacetate + NADH
Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
L-aspartate = beta-alanine + CO(2)
L-glutamate = 4-aminobutanoate + CO(2)
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH
6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO(2) + NADPH
Acetyl-CoA + an N-hydroxyarylamine = CoA + an N-acetoxyarylamine
(1a) N(6)-(3-methylbut-2-en-1-yl)-adenine(37) in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = N(6)-(3-methylbut-2-en-1-yl)-2-thioadenine(37) in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor
Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside
ATP + acetate = ADP + acetyl phosphate
(S)-malate = fumarate + H(2)O
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H(2)O
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Vanillyl alcohol + O(2) = vanillin + H(2)O(2)
Isocitrate = succinate + glyoxylate
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Isovaleryl-CoA + electron-transfer flavoprotein = 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol
Hydrolysis of terminal non-reducing alpha-L-rhamnose residues in alpha-L-rhamnosides
Preferential release of a C-terminal lysine or arginine amino acid.
Catechol + O(2) = cis,cis-muconate
10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
An aldehyde + NAD(P)(+) + H(2)O = a carboxylate + NAD(P)H
Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product
Taurine + 2-oxoglutarate + O(2) = sulfite + aminoacetaldehyde + succinate + CO(2)
Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides
(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH
Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
CoB + CoM + methanophenazine = CoM-S-S-CoB + dihydromethanophenazine
(1a) 2 L-ascorbate + H(2)O(2) + 2 H(+) = 2 monodehydroascorbate + 2 H(2)O
6-phospho-D-glucono-1,5-lactone + H(2)O = 6-phospho-D-gluconate
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(79) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(79)
1D-chiro-inositol + NAD(+) = 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH
Produces alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride (DFA I) by successively eliminating the diminishing (2->1)-beta-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide.
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in (1->6)-alpha-D-glucans and derived oligosaccharides
L-alanine = D-alanine
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu)
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)(+) + H(2)O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H
Chorismate = prephenate
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH
GTP = 3',5'-cyclic GMP + diphosphate
3 malonyl-CoA + benzoyl-CoA = 4 CoA + 3,5-dihydroxybiphenyl + 4 CO(2)
Myo-inositol + NAD(+) = 2,4,6/3,5-pentahydroxycyclohexanone + NADH
RH + Cl(-) + H(2)O(2) = RCl + 2 H(2)O
2-alpha-D-glucosyl-D-glucose + phosphate = D-glucose + beta-D-glucose 1-phosphate
ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD(+)
ATP + (d)CMP = ADP + (d)CDP
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
ATP + L-aspartate = ADP + 4-phospho-L-aspartate
(1a) glutaryl-CoA + electron-transfer flavoprotein = (E)-glutaconyl-CoA + reduced electron-transfer flavoprotein
A 3-oxosteroid + acceptor = a 3-oxo-Delta(1)-steroid + reduced acceptor
ATP = 3',5'-cyclic AMP + diphosphate
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanoyl-CoA + propanoyl-CoA = CoA + 3-alpha,7-alpha,12-alpha-trihydroxy-24-oxo-5-beta-cholestanoyl-CoA
Myristoyl-CoA + acetyl-CoA = 3-oxopalmitoyl-CoA + CoA
ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide
Acetyl-CoA + formate = CoA + pyruvate
Alpha-maltose 1-phosphate + ((1->4)-alpha-D-glucosyl)(n) = phosphate + ((1->4)-alpha-D-glucosyl)(n+2)
Formylmethanofuran + H(2)O + 2 oxidized ferredoxin [iron-sulfur] cluster = CO(2) + methanofuran + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+)
UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1)
Similar to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
(S)-mandelate = (R)-mandelate
NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5
Maleate = fumarate
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)
(S)-2-haloacid + H(2)O = (R)-2-hydroxyacid + halide
Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.
2-hydroxy-2H-chromene-2-carboxylate = (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate
Prephenate + NAD(+) = 4-hydroxyphenylpyruvate + CO(2) + NADH
Meso-2,6-diaminoheptanedioate + H(2)O + NADP(+) = L-2-amino-6-oxoheptanedioate + NH(3) + NADPH
Isopentenyl diphosphate = prenyl diphosphate
ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole 
S-adenosyl-L-methionine + cytidine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(1402) in 16S rRNA 
ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
(1a) [enzyme]-L-histidine + 2,3-bisphospho-D-glycerate = [enzyme]-N(tau)-phospho-L-histidine + 2/3-phospho-D-glycerate
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
L-aspartate = fumarate + NH(3)
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO(2)
Orotidine 5'-phosphate = UMP + CO(2)
Anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate
N-acylsphingosine + H(2)O = a carboxylate + sphingosine
Coproporphyrinogen-III + O(2) + 2 H(+) = protoporphyrinogen-IX + 2 CO(2) + 2 H(2)O
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
Adenosine + H(2)O = inosine + NH(3)
GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H(2)O
Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate
All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate
(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)
L-lysine = cadaverine + CO(2)
L-erythro-3,5-diaminohexanoate + H(2)O + NAD(+) = (S)-5-amino-3-oxohexanoate + NH(3) + NADH
H(2) + oxidized coenzyme F420 = reduced coenzyme F420
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine
(R)-10-hydroxystearate = oleate + H(2)O
N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate
Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate
Acetyl-CoA + H(2)O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
An acyl-[acyl-carrier-protein] + S-adenosyl-L-methionine = [acyl-carrier-protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
2-iminobutanoate + H(2)O = 2-oxobutanoate + NH(3)
ATP + D-fructose = ADP + D-fructose 6-phosphate
Coproporphyrinogen III + 3 O(2) = coproporphyrin III + 3 H(2)O(2)
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
4-maleylacetoacetate = 4-fumarylacetoacetate
n UDP-alpha-D-glucose + 4-O-(poly((2R)-glycerophospho)-(2R)-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = n UDP + 4-O-(poly((2R)-2-alpha-D-glucosyl-1-glycerophospho)-(2R)-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose
CTP + N-acylneuraminate = diphosphate + CMP-N-acylneuraminate
(S)-3-hydroxy-3-methylglutaryl-CoA = acetyl-CoA + acetoacetate
NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone
sn-glycerol 3-phosphate + O(2) = glycerone phosphate + H(2)O(2)
(S)-ureidoglycolate = glyoxylate + urea
L-lysine + O(2) = 5-aminopentanamide + CO(2) + H(2)O
ATP + thiamine = AMP + thiamine diphosphate
ATP + H(2)O + H(+)(Side 1) = ADP + phosphate + H(+)(Side 2)
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.
ATP + H(2)O + H(+)(Side 1) + K(+)(Side 2) = ADP + phosphate + H(+)(Side 2) + K(+)(Side 1)
(R)-mevalonate + CoA + 2 NAD(+) = 3-hydroxy-3-methylglutaryl-CoA + 2 NADH
Protein L-glutamine + H(2)O = protein L-glutamate + NH(3)
N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H(2)O = L-2-amino acid + NH(3) + CO(2)
Glutarate + 2-oxoglutarate + O(2) = (S)-2-hydroxyglutarate + succinate + CO(2)
2 glutathione + NADP(+) = glutathione disulfide + NADPH
A nitroalkane + H(2)O + O(2) = an aldehyde or ketone + nitrite + H(2)O(2)
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H(2)O = 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH
L-alanine + 3-oxopropanoate = pyruvate + beta-alanine
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD(+) = 2-methylacetoacetyl-CoA + NADH
Nitrous oxide + 2 ferricytochrome c + H(2)O = 2 nitric oxide + 2 ferrocytochrome c + 2 H(+)
L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde
ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
ATP + 3'-dephospho-CoA = ADP + CoA
2 glutathione + ROOH = glutathione disulfide + H(2)O + ROH
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate
Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+)
Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]
Hydroxymethylbilane = uroporphyrinogen III + H(2)O
L-histidine-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-((3S)-3-amino-3-carboxypropyl)-L-histidine-[translation elongation factor 2] + S-methyl-5'-thioadenosine
CTP + phosphocholine = diphosphate + CDP-choline
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
ATP + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine = ADP + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine
(1a) S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1
ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate
Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
N(6)-(Delta(2)-isopentenyl)-adenosine 5'-phosphate + H(2)O = N(6)-(dimethylallyl)adenine + D-ribose 5'-phosphate
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-galactosamine
(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide
Acetoacetate = acetone + CO(2)
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate
Glycerol = 3-hydroxypropanal + H(2)O
[Molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine + reduced acceptor = AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + [cysteine desulfurase] + oxidized acceptor
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Acetyl-CoA + D-glucosamine 6-phosphate = CoA + N-acetyl-D-glucosamine 6-phosphate
Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
ADP-D-ribose + H(2)O = AMP + D-ribose 5-phosphate
ATP + thiamine phosphate = ADP + thiamine diphosphate
ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H(2)O = 5-formyl-5,6,7,8-tetrahydromethanopterin
Chlorophyllide a + NADP(+) = protochlorophyllide + NADPH
(2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO(2)
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Reduced riboflavin + NADP(+) = riboflavin + NADPH
Hydrolysis of the bonds linking certain hydrophobic residues in hemoglobin or globin. Also cleaves small molecules substrates such as Ala-Leu-Glu-Arg-Thr-Phe-|-Phe(NO(2))-Ser-Phe-Pro-Thr.
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
NADPH + 2 quinone = NADP(+) + 2 semiquinone
Cinnamyl alcohol + NADP(+) = cinnamaldehyde + NADPH
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione
ATP + riboflavin = ADP + FMN
2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate
Phenylacetaldehyde + NAD(+) + H(2)O = phenylacetate + NADH
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
1-(5-phospho-beta-D-ribosyl)-AMP + H(2)O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide
UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6(F)-phosphate
UDP-N-acetyl-alpha-D-glucosamine + H(2)O = N-acetyl-D-mannosamine + UDP
Meso-2,6-diaminoheptanedioate = L-lysine + CO(2)
ATP + FMN = diphosphate + FAD
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
1-(5-phospho-beta-D-ribosyl)-ATP + H(2)O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate
Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.
5-carboxymethyl-2-hydroxymuconate = (3E,5R)-5-carboxy-2-oxohept-3-enedioate
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.
Arachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in amylaceous polysaccharides, to remove successive maltohexaose residues from the non-reducing chain ends
ATP + acetate + CoA = ADP + phosphate + acetyl-CoA
ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-bisphosphate
Preferential cleavage of bonds with hydrophobic residues in P1'.
Protein dithiol + NAD(P)(+) = protein disulfide + NAD(P)H
2 R'C(R)SH + O(2) = R'C(R)S-S(R)CR' + H(2)O(2)
5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NADPH
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
ATP + (phosphate)(n) = ADP + (phosphate)(n+1)
Hydrolysis of terminal, non-reducing beta-D-galactose residues in (1->3)-beta-D-galactopyranans
UDP-N-acetyl-alpha-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1)
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + H(2)O = 5-amino-6-(5-phosphoribosylamino)uracil + NH(3)
S-adenosyl-L-methionine + ethanolamine phosphate = S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans
A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)
10-formyltetrahydrofolate + NADP(+) + H(2)O = tetrahydrofolate + CO(2) + NADPH
Penicillin + H(2)O = a carboxylate + 6-aminopenicillanate
D-xylopyranose = D-xylulose
A long-chain aldehyde + O(2) + 2 NADPH = an alkane + formate + H(2)O + 2 NADP(+)
NAD(+) + H(2)O = ADP-D-ribose + nicotinamide
Hydrolyzes poly(ADP-D-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-D-ribose
A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine
S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical
An aromatic primary alcohol + O(2) = an aromatic aldehyde + H(2)O(2)
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H(2)O = CoA + N-succinyl-L-2-amino-6-oxoheptanedioate
Release of an N-terminal D-amino acid from a peptide, Xaa-|-Yaa-, in which Xaa is preferably D-Ala, D-Ser or D-Thr. D-amino acid amides and methyl esters also are hydrolyzed, as is glycine amide.
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
Nitrogen + H(2)O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H(+)
S-adenosyl-L-methionine + a 5-hydroxyfurocoumarin = S-adenosyl-L-homocysteine + a 5-methoxyfurocoumarin
ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains
ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione
GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + H(2)O = GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + D-alanine
Pectin + n H(2)O = n methanol + pectate
(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH
3-oxoadipate + NAD(P)(+) = 2-maleylacetate + NAD(P)H
Hydroxypyruvate = 2-hydroxy-3-oxopropanoate
NADH + a quinone = NAD(+) + a quinol
4-aminobutanal + NAD(+) + H(2)O = 4-aminobutanoate + NADH
ATP + benzoate + CoA = AMP + diphosphate + benzoyl-CoA
(2S)-2-hydroxy-3,4-dioxopentyl phosphate = 3-hydroxy-2,4-dioxopentyl phosphate
S-adenosyl-L-methionine + (S)-scoulerine = S-adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose
Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine
sn-glycerol 3-phosphate + NAD(+) = glycerone phosphate + NADH
Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor.
Phylloquinol + a protein with a disulfide bond = phylloquinone + a protein with reduced L-cysteine residues
Putrescine + O(2) + H(2)O = 4-aminobutanal + NH(3) + H(2)O(2)
Endohydrolysis of (1->4)-beta-D-galactosidic linkages in blood group A and B substances
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H(2)O
UDP-alpha-D-glucuronate + NAD(+) = UDP-beta-L-threo-pentapyranos-4-ulose + CO(2) + NADH
Random hydrolysis of (1->2)-glucosidic linkages in (1->2)-beta-D-glucans
Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate
4-amino-5-aminomethyl-2-methylpyrimidine + H(2)O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia
2'-deoxyuridine + phosphate = uracil + 2-deoxy-alpha-D-ribose 1-phosphate
(1a) (R)-6-hydroxynicotine + O(2) = 5-(N-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridin-2-ol + H(2)O(2)
ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA
3'-phosphoadenylyl sulfate + alpha,alpha-trehalose = adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose
10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinose = 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinose
ATP + L-arginine = ADP + N(omega)-phospho-L-arginine
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO(2)
Sucrose 6(F)-phosphate + H(2)O = sucrose + phosphate
(R)-lactate + N-acetyl-D-glucosamine 6-phosphate = N-acetylmuramate 6-phosphate + H(2)O
Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
An aryl sulfate + a phenol = a phenol + an aryl sulfate
N-acetyl-D-glucosamine 6-phosphate + H(2)O = D-glucosamine 6-phosphate + acetate
4-hydroxy-3-methoxyphenyl-beta-hydroxypropanoyl-CoA = feruloyl-CoA + H(2)O
N(2)-acetyl-L-ornithine + H(2)O = acetate + L-ornithine
O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
D-allothreonine = glycine + acetaldehyde
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP
Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
(1a) 2-oxo-3-sulfanylpropanoate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine = pyruvate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
A short-chain (3S)-3-hydroxyacyl-CoA = a short-chain trans-2-enoyl-CoA + H(2)O
Phosphoenolpyruvate + glycerone = pyruvate + glycerone phosphate
(3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H(2)O
O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate
L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate
3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH
7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
Preferential cleavage with hydrophobic residues at P2, P1 and P1'.
Formyl-CoA + oxalate = formate + oxalyl-CoA
Acyl-CoA + NAD(+) = trans-didehydroacyl-CoA + NADH
Glutaredoxin + ROOH = glutaredoxin disulfide + H(2)O + ROH
R-CHOH-R' + NADP(+) = R-CO-R' + NADPH
(1a) L-serine = 2-aminoprop-2-enoate + H(2)O
L-erythrulose 1-phosphate = D-erythrulose 4-phosphate
D-glucose + NAD(P)(+) = D-glucono-1,5-lactone + NAD(P)H
2-deoxy-D-ribosyl-base(1) + base(2) = 2-deoxy-D-ribosyl-base(2) + base(1) 
L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H(2)O
Endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran
Chitin + H(2)O = chitosan + acetate
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
An alpha-L-fucoside + H(2)O = L-fucose + an alcohol
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H(2)O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Thiosulfate + cyanide = sulfite + thiocyanate
ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate
Myo-inositol phosphate + H(2)O = myo-inositol + phosphate
Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 3'-phosphate
3-phospho-D-glycerate + NAD(+) = 3-phosphonooxypyruvate + NADH
(1a) ATP + (R)-lipoate = lipoyl-AMP + diphosphate
[Amino group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H(2)O = [amino group carrier protein]-C-terminal-L-glutamate + L-lysine
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
A short-chain acyl-CoA + electron-transfer flavoprotein = a short-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein
2-phosphoglycolate + H(2)O = glycolate + phosphate
Cyclohexanone + NADPH + O(2) = hexano-6-lactone + NADP(+) + H(2)O
5,6-dihydrouracil + H(2)O = 3-ureidopropanoate
A 3'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO(2)
Xylitol + NADP(+) = L-xylulose + NADPH
3-beta-D-glucosyl-D-glucose + phosphate = D-glucose + alpha-D-glucose 1-phosphate
Hydrolysis of the 1,3-alpha-L-galactosidic linkages of neoagaro-oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L-galactose and D-galactose
(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate
4-coumarate = 4-vinylphenol + CO(2)
ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate
Phosphoenolpyruvate + N-acetyl-D-mannosamine + H(2)O = phosphate + N-acetylneuraminate
Acetyl-CoA + phosphate = CoA + acetyl phosphate
Acetyl-CoA + maltose = CoA + acetyl-maltose
Succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO(2)
Beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-L-serine/L-threonine-[protein] + H(2)O = beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosamine + L-serine/L-threonine-[protein]
Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans
Release of a C-terminal amino acid with broad specificity, except for -Pro.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
ATP + D-gluconate = ADP + 6-phospho-D-gluconate
Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O = (2S)-2-isopropylmalate + CoA
Alpha-D-glucose = beta-D-glucose
GDP-mannose + 3-phospho-D-glycerate = GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
The primary cleavage site is at 67-His-|-Lys-68 in human C5a with a minor secondary cleavage site at 58-Ala-|-Ser-59
ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate
((1->2)-beta-D-glucosyl)(n) + H(2)O = sophorose + ((1->2)-beta-D-glucosyl)(n-2)
(S)-lactaldehyde + NAD(+) + H(2)O = (S)-lactate + NADH
A pyrimidine 5'-nucleotide + H(2)O = D-ribose 5-phosphate + a pyrimidine base
CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO(2) + H(2)O
5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine
5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate
Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO(2)
UDP-N,N'-diacetylbacillosamine + tritrans,heptacis-undecaprenyl phosphate = UMP + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate
Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO(2) + holo-[acyl-carrier protein]
Acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein]
S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol
Succinate semialdehyde + NADP(+) + H(2)O = succinate + NADPH
Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
(3Z)-phytochromobilin + 2 oxidized [2Fe-2S] ferredoxin = biliverdin IX-alpha + 2 reduced [2Fe-2S] ferredoxin
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
L-arabinose = L-ribulose
(1a) a nucleoside 5'-triphosphate + H(2)O = a nucleoside 5'-diphosphate + phosphate
S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(32) in tRNA
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = 8 diphosphate + di-trans,octa-cis-undecaprenyl diphosphate
Cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + H(2)O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]
ATP + UMP = ADP + UDP
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
ATP + nicotinamide ribonucleotide = diphosphate + NAD(+)
Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)
Sedoheptulose 1,7-bisphosphate + H(2)O = sedoheptulose 7-phosphate + phosphate
RH + H(2)O(2) = ROH + H(2)O
2-deoxy-D-gluconate + NAD(+) = 3-dehydro-2-deoxy-D-gluconate + NADH
ATP + H(2)O + vitamin B12-[cobalamin-binding protein](Side 1) = ADP + phosphate + vitamin B12(Side 2) + [cobalamin-binding protein](Side 1)
L-threonine = glycine + acetaldehyde
Alditol + NAD(P)(+) = aldose + NAD(P)H
ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr)
D-glucuronate = D-fructuronate
Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
8-oxo-dGTP + H(2)O = 8-oxo-dGMP + diphosphate
Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan
D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H(2)O
Arsenate + glutathione + glutaredoxin = arsenite + a glutaredoxin-glutathione disulfide + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 45-mer (preferred)
Entry contents:
42 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (45 distinct):
Ribosomal_L2_C domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 260 amino acids
Theoretical weight: 28.06 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I3T9 (Residues: 1-260; Coverage: 100%)
Gene name: PF3D7_0516900
Sequence domains:
60S ribosomal protein L3 Chain: E
Molecule details ›
Chain: E
Length: 386 amino acids
Theoretical weight: 44.32 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IJC6 (Residues: 1-386; Coverage: 100%)
Gene name: PF3D7_1027800
Sequence domains: Ribosomal protein L3
Ribos_L4_asso_C domain-containing protein Chain: F
Molecule details ›
Chain: F
Length: 411 amino acids
Theoretical weight: 46.31 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I431 (Residues: 1-411; Coverage: 100%)
Gene name: PF3D7_0507100
Sequence domains:
60S ribosomal protein L11a, putative Chain: G
Molecule details ›
Chain: G
Length: 173 amino acids
Theoretical weight: 20.27 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IBQ6 (Residues: 1-173; Coverage: 100%)
Gene name: PF3D7_0719600
Sequence domains:
60S ribosomal protein L6, putative Chain: H
Molecule details ›
Chain: H
Length: 190 amino acids
Theoretical weight: 21.64 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IE85 (Residues: 1-190; Coverage: 100%)
Gene name: PF3D7_1323100
Sequence domains: Ribosomal protein L6
60S ribosomal protein eL6 Chain: I
Molecule details ›
Chain: I
Length: 221 amino acids
Theoretical weight: 25.59 KDa
Source organism: Plasmodium falciparum 3D7
60S ribosomal protein L7a Chain: J
Molecule details ›
Chain: J
Length: 283 amino acids
Theoretical weight: 32.76 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8ILL2 (Residues: 1-283; Coverage: 100%)
Gene name: PF3D7_1424400
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
60S ribosomal protein L13, putative Chain: K
Molecule details ›
Chain: K
Length: 202 amino acids
Theoretical weight: 23.81 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IJZ7 (Residues: 1-202; Coverage: 100%)
Gene name: PF3D7_1004000
Sequence domains: Ribosomal protein L13
60S ribosomal protein L13 Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 25.51 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IAX6 (Residues: 1-215; Coverage: 100%)
Gene name: PF3D7_0814000
Sequence domains: Ribosomal protein L13e
60S ribosomal protein L23, putative Chain: M
Molecule details ›
Chain: M
Length: 139 amino acids
Theoretical weight: 15.02 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IE09 (Residues: 1-139; Coverage: 100%)
Gene name: PF3D7_1331800
Sequence domains: Ribosomal protein L14p/L23e
Ribosomal_L14e domain-containing protein Chain: N
Molecule details ›
Chain: N
Length: 165 amino acids
Theoretical weight: 19.34 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8ILE8 (Residues: 1-165; Coverage: 100%)
Gene name: PF3D7_1431700
Sequence domains: Ribosomal protein L14
Ribosomal_L18e/L15P domain-containing protein Chain: O
Molecule details ›
Chain: O
Length: 148 amino acids
Theoretical weight: 16.77 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C6KT23 (Residues: 1-148; Coverage: 100%)
Gene name: PF3D7_0618300
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Ribosomal protein L15 Chain: P
Molecule details ›
Chain: P
Length: 205 amino acids
Theoretical weight: 24.2 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C0H4A6 (Residues: 1-205; Coverage: 100%)
Gene name: PF3D7_0415900
Sequence domains: Ribosomal L15
Ribosomal_L16 domain-containing protein Chain: Q
Molecule details ›
Chain: Q
Length: 219 amino acids
Theoretical weight: 25.27 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8ILV2 (Residues: 1-219; Coverage: 100%)
Gene name: PF3D7_1414300
Sequence domains: Ribosomal protein L16p/L10e
Ribosomal_L18_c domain-containing protein Chain: R
Molecule details ›
Chain: R
Length: 294 amino acids
Theoretical weight: 34.07 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8ILL3 (Residues: 1-294; Coverage: 100%)
Gene name: PF3D7_1424100
Sequence domains:
Ribosomal_L18e/L15P domain-containing protein Chain: S
Molecule details ›
Chain: S
Length: 187 amino acids
Theoretical weight: 21.51 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C0H5G3 (Residues: 1-187; Coverage: 100%)
Gene name: PF3D7_1341300
Sequence domains: Ribosomal protein 60S L18 and 50S L18e
Ribosomal_L19e domain-containing protein Chain: T
Molecule details ›
Chain: T
Length: 182 amino acids
Theoretical weight: 21.64 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C6KSY6 (Residues: 1-182; Coverage: 100%)
Gene name: PF3D7_0614500
Sequence domains: Ribosomal protein L19e
60S ribosomal protein L18a Chain: U
Molecule details ›
Chain: U
Length: 184 amino acids
Theoretical weight: 21.79 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IDS6 (Residues: 1-184; Coverage: 100%)
Gene name: PF3D7_1341200
Sequence domains: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
60S ribosomal protein L21 Chain: V
Molecule details ›
Chain: V
Length: 161 amino acids
Theoretical weight: 18.84 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8ILK3 (Residues: 1-161; Coverage: 100%)
Gene name: PF3D7_1426000
Sequence domains: Ribosomal protein L21e
60S ribosomal protein L17, putative Chain: W
Molecule details ›
Chain: W
Length: 203 amino acids
Theoretical weight: 23.47 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IDI5 (Residues: 1-203; Coverage: 100%)
Gene name: PF3D7_1351400
Sequence domains: Ribosomal protein L22p/L17e
60S ribosomal protein L22, putative Chain: X
Molecule details ›
Chain: X
Length: 139 amino acids
Theoretical weight: 16.42 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IB51 (Residues: 1-139; Coverage: 100%)
Gene name: PF3D7_0821700
Sequence domains: Ribosomal L22e protein family
Ribosomal_L23eN domain-containing protein Chain: Y
Molecule details ›
Chain: Y
Length: 190 amino acids
Theoretical weight: 22.15 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IE82 (Residues: 1-190; Coverage: 100%)
Gene name: PF3D7_1323400
Sequence domains:
KOW domain-containing protein Chain: Z
Molecule details ›
Chain: Z
Length: 126 amino acids
Theoretical weight: 14.86 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: O77364 (Residues: 1-126; Coverage: 100%)
Gene name: PF3D7_0312800
Sequence domains:
60S ribosomal protein L24, putative Chain: 0
Molecule details ›
Chain: 0
Length: 162 amino acids
Theoretical weight: 19.3 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IEM3 (Residues: 1-162; Coverage: 100%)
Gene name: PF3D7_1309100
Sequence domains: Ribosomal protein L24e
60S ribosomal protein L27 Chain: 1
Molecule details ›
Chain: 1
Length: 146 amino acids
Theoretical weight: 16.79 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IKM5 (Residues: 1-146; Coverage: 100%)
Gene name: PF3D7_1460700
Sequence domains: Ribosomal L27e protein family
Ribosomal_L28e domain-containing protein Chain: 2
Molecule details ›
Chain: 2
Length: 127 amino acids
Theoretical weight: 14.44 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IHU0 (Residues: 1-127; Coverage: 100%)
Gene name: PF3D7_1142500
Sequence domains: Ribosomal L28e protein family
60S ribosomal protein L35, putative Chain: 3
Molecule details ›
Chain: 3
Length: 124 amino acids
Theoretical weight: 14.79 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IIB4 (Residues: 1-124; Coverage: 100%)
Gene name: PF3D7_1124900
Sequence domains: Ribosomal L29 protein
60S ribosomal protein L29 Chain: 4
Molecule details ›
Chain: 4
Length: 67 amino acids
Theoretical weight: 8.04 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C6S3J6 (Residues: 1-67; Coverage: 100%)
Gene name: PF3D7_1460300
Sequence domains: Ribosomal L29e protein family
60S ribosomal protein L7, putative Chain: 5
Molecule details ›
Chain: 5
Length: 257 amino acids
Theoretical weight: 30.59 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: O97250 (Residues: 1-257; Coverage: 100%)
Gene name: PF3D7_0307200
Sequence domains:
Ribosomal_L7Ae domain-containing protein Chain: 6
Molecule details ›
Chain: 6
Length: 108 amino acids
Theoretical weight: 11.79 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IJK8 (Residues: 1-108; Coverage: 100%)
Gene name: PF3D7_1019400
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
60S ribosomal protein L31 Chain: 7
Molecule details ›
Chain: 7
Length: 120 amino acids
Theoretical weight: 14.1 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I463 (Residues: 1-120; Coverage: 100%)
Gene name: PF3D7_0503800
Sequence domains: Ribosomal protein L31e
60S ribosomal protein L32 Chain: 8
Molecule details ›
Chain: 8
Length: 131 amino acids
Theoretical weight: 15.57 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I3B0 (Residues: 1-131; Coverage: 100%)
Gene name: PF3D7_0903900
Sequence domains: Ribosomal protein L32
60S ribosomal protein L35ae, putative Chain: 9
Molecule details ›
Chain: 9
Length: 140 amino acids
Theoretical weight: 16.31 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IHT9 (Residues: 1-140; Coverage: 100%)
Gene name: PF3D7_1142600
Sequence domains: Ribosomal protein L35Ae
60S ribosomal protein L34 Chain: a
Molecule details ›
Chain: a
Length: 150 amino acids
Theoretical weight: 17.41 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IBY4 (Residues: 1-150; Coverage: 100%)
Gene name: PF3D7_0710600
Sequence domains: Ribosomal protein L34e
60S ribosomal protein L36 Chain: b
Molecule details ›
Chain: b
Length: 112 amino acids
Theoretical weight: 12.83 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I713 (Residues: 1-112; Coverage: 100%)
Gene name: PF3D7_1109900
Sequence domains: Ribosomal protein L36e
Ribosomal protein L37 Chain: c
Molecule details ›
Chain: c
Length: 92 amino acids
Theoretical weight: 10.57 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C0H4L5 (Residues: 1-92; Coverage: 100%)
Gene name: PF3D7_0706400
Sequence domains: Ribosomal protein L37e
60S ribosomal protein L38 Chain: d
Molecule details ›
Chain: d
Length: 87 amino acids
Theoretical weight: 10.34 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8II62 (Residues: 1-87; Coverage: 100%)
Gene name: PF3D7_1130100
Sequence domains: Ribosomal L38e protein family
60S ribosomal protein L39 Chain: e
Molecule details ›
Chain: e
Length: 51 amino acids
Theoretical weight: 6.54 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C0H4H3 (Residues: 1-51; Coverage: 100%)
Gene name: PF3D7_0611700
Sequence domains: Ribosomal L39 protein
Ubiquitin-like domain-containing protein Chain: f
Molecule details ›
Chain: f
Length: 128 amino acids
Theoretical weight: 14.65 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8ID50 (Residues: 1-128; Coverage: 100%)
Gene name: PF3D7_1365900
Sequence domains:
60S ribosomal protein L41 Chain: g
Molecule details ›
Chain: g
Length: 39 amino acids
Theoretical weight: 5.11 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C6S3G4 (Residues: 1-39; Coverage: 100%)
Gene name: PF3D7_1144300
Sequence domains: Ribosomal protein L41
60S ribosomal protein L37a Chain: h
Molecule details ›
Chain: h
Length: 96 amino acids
Theoretical weight: 10.83 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: O96184 (Residues: 1-96; Coverage: 100%)
Gene names: PFB0455w, RPL37A
Sequence domains: Ribosomal L37ae protein family
60S ribosomal protein L44 Chain: i
Molecule details ›
Chain: i
Length: 104 amino acids
Theoretical weight: 12.19 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: O97231 (Residues: 1-104; Coverage: 100%)
Gene names: MAL3P2.9, PFC0200W, RPL44
Sequence domains: Ribosomal protein L44
28S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 3788 nucleotides
Theoretical weight: 1216.21 KDa
Sequence domains:
  • Archaeal large subunit ribosomal RNA
  • Bacterial large subunit ribosomal RNA
  • Eukaryotic large subunit ribosomal RNA
  • 5S ribosomal RNA Chain: B
    Molecule details ›
    Chain: B
    Length: 119 nucleotides
    Theoretical weight: 38.41 KDa
    Sequence domains: 5S ribosomal RNA
    5.8S ribosomal RNA Chain: C
    Molecule details ›
    Chain: C
    Length: 159 nucleotides
    Theoretical weight: 51.21 KDa
    Sequence domains: 5.8S ribosomal RNA

    Ligands and Environments

    2 bound ligands:
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    Resolution: 3.2Å
    Relevant EMDB volumes: EMD-2660