3j4r

Electron Microscopy
35Å resolution

Pseudo-atomic model of the AKAP18-PKA Complex in a linear conformation derived from electron microscopy

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
A-kinase anchor protein 7 isoforms delta and gamma Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 39.48 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6JP77 (Residues: 1-353; Coverage: 100%)
Gene names: Akap18, Akap7
Sequence domains:
cAMP-dependent protein kinase type II-alpha regulatory subunit Chains: B, C
Molecule details ›
Chains: B, C
Length: 402 amino acids
Theoretical weight: 45.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12367 (Residues: 1-21, 22-401; Coverage: 100%)
Gene name: Prkar2a
Sequence domains:
cAMP-dependent protein kinase catalytic subunit alpha Chains: D, E
Molecule details ›
Chains: D, E
Length: 351 amino acids
Theoretical weight: 40.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05132 (Residues: 1-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 35Å
Relevant EMDB volumes: EMD-5756
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)