› 3j4r

Electron Microscopy
35Å resolution

Pseudo-atomic model of the AKAP18-PKA Complex in a linear conformation derived from electron microscopy

Released:
DOI: 10.2210/pdb3j4r/pdb
PDB entry 3j4r coloured by chain, front view.
PDB entry 3j4r coloured by chain, side view.
PDB entry 3j4r coloured by chain, top view.
Source organisms:
Primary publication:
Intrinsic disorder within an AKAP-protein kinase A complex guides local substrate phosphorylation.
Smith FD, Reichow SL, Esseltine JL, Shi D, Langeberg LK, Scott JD, Gonen T
Elife 2 e01319 (2013)
PMID: 24192038
Related structures: EMD-5756

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-138550 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
A-kinase anchor protein 7 isoforms delta and gamma Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 39.48 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6JP77 (Residues: 1-353; Coverage: 100%)
Gene names: Akap18, Akap7
Sequence domains:
cAMP-dependent protein kinase type II-alpha regulatory subunit Chains: B, C
Molecule details ›
Chains: B, C
Length: 402 amino acids
Theoretical weight: 45.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12367 (Residues: 1-21, 22-401; Coverage: 100%)
Gene name: Prkar2a
Sequence domains:
cAMP-dependent protein kinase catalytic subunit alpha Chains: D, E
Molecule details ›
Chains: D, E
Length: 351 amino acids
Theoretical weight: 40.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05132 (Residues: 1-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 35Å
Relevant EMDB volumes: EMD-5756
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Signalling scaffolds and local organization of cellular behaviour.
Langeberg et al. (2015)
17 more

3 mentions without citation

Therapeutic strategies for anchored kinases and phosphatases: exploiting short linear motifs and intrinsic disorder.
Nygren et al. (2015)
2 more