3j0q

Electron Microscopy
10.6Å resolution

Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 10.6A cryo-em map: rotated PRE state 2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
Entry contents:
6 distinct polypeptide molecules
14 distinct RNA molecules
Macromolecules (20 distinct):
Ribosomal protein S15 Chain: S
Molecule details ›
Chain: S
Length: 125 amino acids
Theoretical weight: 14.03 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein S23 Chain: L
Molecule details ›
Chain: L
Length: 141 amino acids
Theoretical weight: 15.65 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein S30 Chain: X
Molecule details ›
Chain: X
Length: 68 amino acids
Theoretical weight: 7.89 KDa
Source organism: Oryctolagus cuniculus
60S ribosomal protein L1-A Chain: B
Molecule details ›
Chain: B
Length: 213 amino acids
Theoretical weight: 24.01 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P0CX43 (Residues: 4-216; Coverage: 98%)
Gene names: RPL1A, SSM1, SSM1A, YPL220W
Sequence domains: Ribosomal protein L1p/L10e family
Ribosomal protein L10 Chain: J
Molecule details ›
Chain: J
Length: 219 amino acids
Theoretical weight: 25.21 KDa
Source organism: Oryctolagus cuniculus
60S ribosomal protein L11-B Chain: k
Molecule details ›
Chain: k
Length: 165 amino acids
Theoretical weight: 18.78 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: Q3E757 (Residues: 7-171; Coverage: 95%)
Gene names: RP39B, RPL11B, RPL16B, YGR085C
Sequence domains:
40S ribosomal RNA fragment Chain: a
Molecule details ›
Chain: a
Length: 48 nucleotides
Theoretical weight: 15.5 KDa
40S ribosomal RNA fragment Chain: c
Molecule details ›
Chain: c
Length: 17 nucleotides
Theoretical weight: 5.43 KDa
40S ribosomal RNA fragment Chain: d
Molecule details ›
Chain: d
Length: 7 nucleotides
Theoretical weight: 2.28 KDa
40S ribosomal RNA fragment Chain: g
Molecule details ›
Chain: g
Length: 31 nucleotides
Theoretical weight: 9.93 KDa
40S ribosomal RNA fragment Chain: G
Molecule details ›
Chain: G
Length: 13 nucleotides
Theoretical weight: 4.13 KDa
40S ribosomal RNA fragment Chain: f
Molecule details ›
Chain: f
Length: 21 nucleotides
Theoretical weight: 6.79 KDa
40S ribosomal RNA fragment Chain: h
Molecule details ›
Chain: h
Length: 111 nucleotides
Theoretical weight: 35.74 KDa
60S ribosomal RNA fragment Chain: 2
Molecule details ›
Chain: 2
Length: 112 nucleotides
Theoretical weight: 36.1 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
60S ribosomal RNA fragment Chain: 3
Molecule details ›
Chain: 3
Length: 12 nucleotides
Theoretical weight: 3.86 KDa
60S ribosomal RNA fragment Chain: 9
Molecule details ›
Chain: 9
Length: 19 nucleotides
Theoretical weight: 6.11 KDa
60S ribosomal RNA fragment Chain: 7
Molecule details ›
Chain: 7
Length: 50 nucleotides
Theoretical weight: 15.93 KDa
tRNA Chains: W, Y
Molecule details ›
Chains: W, Y
Length: 77 nucleotides
Theoretical weight: 24.8 KDa
Sequence domains: tRNA
Messenger RNA Chain: y
Molecule details ›
Chain: y
Length: 3 nucleotides
Theoretical weight: 873 Da
Messenger RNA Chain: w
Molecule details ›
Chain: w
Length: 2 nucleotides
Theoretical weight: 613 Da

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 10.6Å
Relevant EMDB volumes: EMD-5329