3j0l

Electron Microscopy
9.8Å resolution

Core of mammalian 80S pre-ribosome in complex with tRNAs fitted to a 9.8A cryo-EM map: classic PRE state 1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 32-mer (preferred)
Entry contents:
8 distinct polypeptide molecules
21 distinct RNA molecules
Macromolecules (29 distinct):
Ribosomal protein S5 Chain: T
Molecule details ›
Chain: T
Length: 192 amino acids
Theoretical weight: 21.66 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein S14 Chain: K
Molecule details ›
Chain: K
Length: 140 amino acids
Theoretical weight: 15.22 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein S23 Chain: L
Molecule details ›
Chain: L
Length: 141 amino acids
Theoretical weight: 15.65 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein S30 Chain: X
Molecule details ›
Chain: X
Length: 68 amino acids
Theoretical weight: 7.89 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein S15 Chain: S
Molecule details ›
Chain: S
Length: 125 amino acids
Theoretical weight: 14.03 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein L10a Chain: B
Molecule details ›
Chain: B
Length: 213 amino acids
Theoretical weight: 24.01 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein L10 Chain: J
Molecule details ›
Chain: J
Length: 219 amino acids
Theoretical weight: 25.21 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein L36a Chain: F
Molecule details ›
Chain: F
Length: 95 amino acids
Theoretical weight: 10.96 KDa
Source organism: Oryctolagus cuniculus
40S ribosomal RNA fragment Chain: a
Molecule details ›
Chain: a
Length: 48 nucleotides
Theoretical weight: 15.5 KDa
40S ribosomal RNA fragment Chain: b
Molecule details ›
Chain: b
Length: 12 nucleotides
Theoretical weight: 3.88 KDa
40S ribosomal RNA fragment Chain: c
Molecule details ›
Chain: c
Length: 17 nucleotides
Theoretical weight: 5.43 KDa
40S ribosomal RNA fragment Chain: d
Molecule details ›
Chain: d
Length: 7 nucleotides
Theoretical weight: 2.28 KDa
40S ribosomal RNA fragment Chain: e
Molecule details ›
Chain: e
Length: 4 nucleotides
Theoretical weight: 1.22 KDa
40S ribosomal RNA fragment Chain: E
Molecule details ›
Chain: E
Length: 5 nucleotides
Theoretical weight: 1.48 KDa
40S ribosomal RNA fragment Chain: f
Molecule details ›
Chain: f
Length: 21 nucleotides
Theoretical weight: 6.79 KDa
40S ribosomal RNA fragment Chain: g
Molecule details ›
Chain: g
Length: 31 nucleotides
Theoretical weight: 9.93 KDa
40S ribosomal RNA fragment Chain: G
Molecule details ›
Chain: G
Length: 13 nucleotides
Theoretical weight: 4.13 KDa
40S ribosomal RNA fragment Chain: h
Molecule details ›
Chain: h
Length: 111 nucleotides
Theoretical weight: 35.74 KDa
60S ribosomal RNA fragment Chain: 1
Molecule details ›
Chain: 1
Length: 50 nucleotides
Theoretical weight: 16.04 KDa
60S ribosomal RNA fragment Chain: 2
Molecule details ›
Chain: 2
Length: 112 nucleotides
Theoretical weight: 36.1 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
60S ribosomal RNA fragment Chain: 3
Molecule details ›
Chain: 3
Length: 12 nucleotides
Theoretical weight: 3.86 KDa
60S ribosomal RNA fragment Chain: 4
Molecule details ›
Chain: 4
Length: 14 nucleotides
Theoretical weight: 4.57 KDa
60S ribosomal RNA fragment Chain: 5
Molecule details ›
Chain: 5
Length: 6 nucleotides
Theoretical weight: 1.88 KDa
60S ribosomal RNA fragment Chain: 6
Molecule details ›
Chain: 6
Length: 19 nucleotides
Theoretical weight: 6.22 KDa
60S ribosomal RNA fragment Chain: 7
Molecule details ›
Chain: 7
Length: 50 nucleotides
Theoretical weight: 15.93 KDa
60S ribosomal RNA fragment Chain: 8
Molecule details ›
Chain: 8
Length: 20 nucleotides
Theoretical weight: 6.46 KDa
tRNA Chains: V, W, Y
Molecule details ›
Chains: V, W, Y
Length: 76 nucleotides
Theoretical weight: 24.49 KDa
Sequence domains: tRNA
Messenger RNA Chains: v, y
Molecule details ›
Chains: v, y
Length: 3 nucleotides
Theoretical weight: 873 Da
Messenger RNA Chain: w
Molecule details ›
Chain: w
Length: 2 nucleotides
Theoretical weight: 613 Da

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.8Å
Relevant EMDB volumes: EMD-5326