X-ray diffraction
2.72Å resolution

Homocitrate Synthase Lys4 bound to 2-OG


Function and Biology Details

Reaction catalysed:
Acetyl-CoA + H(2)O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Homocitrate synthase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 423 amino acids
Theoretical weight: 46.75 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
  • Canonical: Q9Y823 (Residues: 1-418; Coverage: 100%)
Gene names: SPBC1105.02c, lys4
Sequence domains: HMGL-like
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P62
Unit cell:
a: 133.798Å b: 133.798Å c: 125.657Å
α: 90° β: 90° γ: 120°
R R work R free
0.18 0.177 0.217
Expression system: Escherichia coli