› 3it7

X-ray diffraction
2.14Å resolution

Crystal Structure of the LasA virulence factor from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb3it7/pdb
PDB entry 3it7 coloured by chain, front view.
PDB entry 3it7 coloured by chain, side view.
PDB entry 3it7 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structure of the LasA virulence factor from Pseudomonas aeruginosa: substrate specificity and mechanism of M23 metallopeptidases.
Spencer J, Murphy LM, Conners R, Sessions RB, Gamblin SJ
J Mol Biol 396 908-23 (2010)
PMID: 20026068

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease LasA Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 19.98 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: P14789 (Residues: 237-418; Coverage: 47%)
Gene names: PA1871, lasA
Sequence domains: Peptidase family M23
Structure domains: Glucose Permease (Domain IIA)

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand TLA 2 x TLA
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 34.964Å b: 58.838Å c: 146.438Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.149 0.195

Quick links

Citations

5 review citations

Peptidoglycan hydrolases-potential weapons against Staphylococcus aureus.
Szweda et al. (2012)
4 more

9 mentions without citation

One fold, many functions-M23 family of peptidoglycan hydrolases.
Razew et al. (2022)
8 more