3isl

X-ray diffraction
2.06Å resolution

Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucG) from Bacillus subtilis

Released:
Source organism: Bacillus subtilis
Entry authors: Costa R, Cendron L, Ramazzina I, Berni R, Peracchi A, Percudani R, Zanotti G

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-ureidoglycine--glyoxylate transaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 416 amino acids
Theoretical weight: 45.8 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O32148 (Residues: 1-416; Coverage: 100%)
Gene names: BSU32520, pucG, yurG
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 102.4Å b: 95.99Å c: 101.29Å
α: 90° β: 114.23° γ: 90°
R-values:
R R work R free
0.196 0.193 0.247
Expression system: Escherichia coli