3iqy

X-ray diffraction
3.3Å resolution

Active site mutants of B. subtilis SecA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein translocase subunit SecA Chain: A
Molecule details ›
Chain: A
Length: 841 amino acids
Theoretical weight: 95.63 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P28366 (Residues: 1-841; Coverage: 100%)
Gene names: BSU35300, div+, secA
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P3112
Unit cell:
a: 131.015Å b: 131.015Å c: 151.966Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.207 0.269
Expression system: Escherichia coli