› 3ipc

X-ray diffraction
1.3Å resolution

Structure of ATU2422-GABA F77A mutant receptor in complex with leucine

Released:
DOI: 10.2210/pdb3ipc/pdb
PDB 3ipc coloured by chain and viewed from the front.
PDB 3ipc coloured by chain and viewed from the side.
PDB 3ipc coloured by chain and viewed from the top.
Source organism: Agrobacterium fabrum str. C58
Primary publication:
A conserved mechanism of GABA binding and antagonism is revealed by structure-function analysis of the periplasmic binding protein Atu2422 in Agrobacterium tumefaciens.
Planamente S, Vigouroux A, Mondy S, Nicaise M, Faure D, Moréra S
J. Biol. Chem. 285 30294-303 (2010)
PMID: 20630861

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine-binding protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 37.44 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7CX36 (Residues: 24-372; Coverage: 100%)
Gene name: Atu2422
Sequence domains: Periplasmic binding protein
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand LEU 1 x LEU
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 119.77Å b: 43.03Å c: 66.46Å
α: 90° β: 109.76° γ: 90°
R-values:
R R work R free
0.159 0.158 0.186
Expression system: Escherichia coli

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Citations

5 review citations

Quorum Sensing and Quorum Quenching in Agrobacterium: A "Go/No Go System"?
Dessaux et al. (2018)
4 more

2 mentions without citation

A conserved mechanism of GABA binding and antagonism is revealed by structure-function analysis of the periplasmic binding protein Atu2422 in Agrobacterium tumefaciens.
Planamente et al. (2010)
1 more