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H57T Hfq from Pseudomonas aeruginosa

X-ray diffraction
1.7Å resolution
PDB entry 3inz coloured by chain, front view.
PDB entry 3inz coloured by chain, side view.
PDB entry 3inz coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Assembly composition:
homo hexamer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: RNA-binding protein Hfq
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Multimeric state: homo hexamer
Accessible surface area: 18408.26 Å2
Buried surface area: 10844.24 Å2
Dissociation area: 4,786.02 Å2
Dissociation energy (ΔGdiss): 66.63 kcal/mol
Dissociation entropy (TΔSdiss): 54.67 kcal/mol
Symmetry number: 6
PDBe Complex ID: PDB-CPX-190968

Macromolecules

RNA-binding protein Hfq
Chains: A, B, C, D, E, F
Length: 82 amino acids
Theoretical weight: 9.08 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HUM0 (Residues: 1-82; Coverage: 100%)
Gene names: PA4944, hfq
Pfam: Hfq protein
InterPro:
CATH: SH3 type barrels.
RNA-binding protein Hfq in PDB entry 3inz, assembly 1, front view.
RNA-binding protein Hfq in PDB entry 3inz, assembly 1, side view.
RNA-binding protein Hfq in PDB entry 3inz, assembly 1, top view.
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