› 3inu

X-ray diffraction
2.5Å resolution

Crystal structure of an unbound KZ52 neutralizing anti-Ebolavirus antibody.

Released:
DOI: 10.2210/pdb3inu/pdb
PDB 3inu coloured by chain and viewed from the front.
PDB 3inu coloured by chain and viewed from the side.
PDB 3inu coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Techniques and tactics used in determining the structure of the trimeric ebolavirus glycoprotein.
Lee JE, Fusco ML, Abelson DM, Hessell AJ, Burton DR, Saphire EO
Acta Crystallogr. D Biol. Crystallogr. 65 1162-80 (2009)
PMID: 19923712

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
KZ52 antibody fragment heavy chain Chains: H, M
Molecule details ›
Chains: H, M
Length: 226 amino acids
Theoretical weight: 24.02 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
KZ52 antibody fragment light chain Chains: L, N
Molecule details ›
Chains: L, N
Length: 217 amino acids
Theoretical weight: 23.9 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3221
Unit cell:
a: 129.52Å b: 129.52Å c: 184.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.203 0.252
Expression system: Cricetulus griseus

Quick links

Citations

3 review citations

Structural Biology Illuminates Molecular Determinants of Broad Ebolavirus Neutralization by Human Antibodies for Pan-Ebolavirus Therapeutic Development.
Murin et al. (2021)
2 more

2 mentions without citation

Techniques and tactics used in determining the structure of the trimeric ebolavirus glycoprotein.
Lee et al. (2009)
1 more