X-ray diffraction
2.28Å resolution

Crystal structure of cAMP-dependent Protein Kinase A Regulatory Subunit I alpha in complex with dual-specific A-Kinase Anchoring Protein 2


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 50 amino acids
Theoretical weight: 6.01 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
  • Canonical: P00514 (Residues: 13-62; Coverage: 13%)
Gene name: PRKAR1A
Sequence domains: Regulatory subunit of type II PKA R-subunit
Structure domains: cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain
A-kinase anchor protein 10, mitochondrial Chain: C
Molecule details ›
Chain: C
Length: 45 amino acids
Theoretical weight: 5.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O43572 (Residues: 623-662; Coverage: 6%)
Gene name: AKAP10

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 40.4Å b: 56.02Å c: 56.99Å
α: 90° β: 90° γ: 90°
R R work R free
0.193 0.186 0.254
Expression system: Escherichia coli