3ij5

X-ray diffraction
1.95Å resolution

1.95 Angstrom Resolution Crystal Structure of 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase from Yersinia pestis

Released:
Source organism: Yersinia pestis CO92
Entry authors: Minasov G, Halavaty A, Shuvalova L, Dubrovska I, Winsor J, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
3-deoxy-D-manno-octulosonate 8-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate + phosphate
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 23.06 KDa
Source organism: Yersinia pestis CO92
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZB47 (Residues: 1-187; Coverage: 100%)
Gene names: YPO3578, YP_3833, kdsC, y0150
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 130.597Å b: 77.941Å c: 96.881Å
α: 90° β: 120.05° γ: 90°
R-values:
R R work R free
0.165 0.163 0.202
Expression system: Escherichia coli