3ign

X-ray diffraction
1.83Å resolution

Crystal Structure of the GGDEF domain from Marinobacter aquaeolei diguanylate cyclase complexed with c-di-GMP - Northeast Structural Genomics Consortium Target MqR89a

Released:

Function and Biology Details

Reaction catalysed:
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GGDEF domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 20.46 KDa
Source organism: Marinobacter hydrocarbonoclasticus VT8
Expression system: Escherichia coli
UniProt:
  • Canonical: A1U3W3 (Residues: 149-316; Coverage: 53%)
Gene name: Maqu_2607
Sequence domains: Diguanylate cyclase, GGDEF domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P43212
Unit cell:
a: 59.229Å b: 59.229Å c: 118.895Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.24
Expression system: Escherichia coli