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X-ray diffraction
2Å resolution

2.0 Angstrom Resolution Crystal Structure of Glucose-6-phosphate Isomerase (pgi) from Bacillus anthracis.

Released:
DOI: 10.2210/pdb3ifs/pdb
PDB 3ifs coloured by chain and viewed from the front.
PDB 3ifs coloured by chain and viewed from the side.
PDB 3ifs coloured by chain and viewed from the top.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Entry authors: Minasov G, Wawrzak Z, Onopriyenko O, Gordon E, Peterson SN, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 453 amino acids
Theoretical weight: 51.1 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81K75 (Residues: 1-450; Coverage: 100%)
Gene names: BAS4767, BA_5130, GBAA_5130, pgi
Sequence domains: Phosphoglucose isomerase
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

4 bound ligands:
Ligand SO4 4 x SO4
Ligand CL 1 x CL
Ligand PGE 1 x PGE
Ligand LI 1 x LI
1 modified residue:
Ligand MSE 54 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21212
Unit cell:
a: 111.802Å b: 303.64Å c: 72.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.14 0.19
Expression system: Escherichia coli

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