3iel

X-ray diffraction
2.35Å resolution

Crystal Structure of TTHA0252 from Thermus thermophilus HB8 complexed with UMP

Released:
Source organism: Thermus thermophilus HB8
Entry authors: Ishikawa H, Nakagawa N, Kuramitsu S, Yokoyama S, Masui R

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease TTHA0252 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 431 amino acids
Theoretical weight: 47.12 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLP1 (Residues: 1-431; Coverage: 100%)
Gene name: TTHA0252
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: C2
Unit cell:
a: 145.238Å b: 146.677Å c: 120.349Å
α: 90° β: 110.11° γ: 90°
R-values:
R R work R free
0.222 0.222 0.259
Expression system: Escherichia coli BL21(DE3)