PDBe 3ie1

X-ray diffraction
2.85Å resolution

Crystal structure of H380A mutant TTHA0252 from Thermus thermophilus HB8 complexed with RNA

Released:
Source organism: Thermus thermophilus HB8
Entry authors: Ishikawa H, Nakagawa N, Kuramitsu S, Yokoyama S, Masui R, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease TTHA0252 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 431 amino acids
Theoretical weight: 47.05 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLP1 (Residues: 1-431; Coverage: 100%)
Gene name: TTHA0252
Sequence domains:
Structure domains:
RNA (5'-R(P*UP*UP*UP*U)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 4 nucleotides
Theoretical weight: 1.18 KDa
Source organism: Thermus thermophilus HB8
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: C2
Unit cell:
a: 143.424Å b: 148.165Å c: 120.837Å
α: 90° β: 109.61° γ: 90°
R-values:
R R work R free
0.231 0.231 0.291
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided