X-ray diffraction
2.7Å resolution

Crystal structure of (102-265)RIIb:C holoenzyme of cAMP-dependent protein kinase


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase type II-beta regulatory subunit Chain: B
Molecule details ›
Chain: B
Length: 164 amino acids
Theoretical weight: 18.74 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
  • Canonical: P12369 (Residues: 102-265; Coverage: 39%)
Gene name: Prkar2b
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 179.272Å b: 67.434Å c: 47.325Å
α: 90° β: 99.51° γ: 90°
R R work R free
0.241 0.241 0.319
Expression system: Escherichia coli