3i6f

X-ray diffraction
1.3Å resolution

Ribonuclease A by Classical hanging drop method before high X-Ray dose on ESRF ID14-2 beamline

Released:
Source organism: Bos taurus
Entry authors: Pechkova E, Tripathi SK, Ravelli R, McSweeney S, Nicolini C

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3221
Unit cell:
a: 64.306Å b: 64.306Å c: 63.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.203 0.22