› 3i5v

X-ray diffraction
2.8Å resolution

Crystal structure of beta toxin 275-280 from Staphylococcus aureus

Released:
DOI: 10.2210/pdb3i5v/pdb
PDB entry 3i5v coloured by chain, front view.
PDB entry 3i5v coloured by chain, side view.
PDB entry 3i5v coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus RN4220
Entry authors: Huseby M, Shi K, Kruse AC, Ohlendorf DH

Function and Biology Details

Reaction catalysed: 
A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase C Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 313 amino acids
Theoretical weight: 35.56 KDa
Source organism: Staphylococcus aureus subsp. aureus RN4220
Expression system: Escherichia coli
UniProt:
  • Canonical: P09978 (Residues: 35-330; Coverage: 99%)
Gene names: hlb, plc
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

1 bound ligand:
Ligand DGA 1 x DGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P1
Unit cell:
a: 55.184Å b: 69.053Å c: 75.654Å
α: 93.06° β: 94.6° γ: 92.13°
R-values:
R R work R free
0.214 0.214 0.267
Expression system: Escherichia coli

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