3i17 › Citations

Elucidating the exact role of engineered CRABPII residues for the formation of a retinal protonated Schiff base.

Vasileiou C, Wang W, Jia X, Lee KS, Watson CT, Geiger JH, Borhan B
Proteins 77 812-22 (2009)
Cited: 11 times
EuropePMC logo PMID: 19603486

Abstract

Cellular Retinoic Acid Binding Protein II (CRABPII) has been reengineered to specifically bind and react with all-trans-retinal to form a protonated Schiff base. Each step of this process has been dissected and four residues (Lys132, Tyr134, Arg111, and Glu121) within the CRABPII binding site have been identified as crucial for imine formation and/or protonation. The precise role of each residue has been examined through site directed mutagenesis and crystallographic studies. The crystal structure of the R132K:L121E-CRABPII (PDB-3I17) double mutant suggests a direct interaction between engineered Glu121 and the native Arg111, which is critical for both Schiff base formation and protonation.

Articles - 3i17 mentioned but not cited (3)

  1. Rotational Symmetry of Two Pyrethroid Receptor Sites in the Mosquito Sodium Channel. Du Y, Nomura Y, Zhorov BS, Dong K. Mol Pharmacol 88 273-280 (2015)
  2. Elucidating the exact role of engineered CRABPII residues for the formation of a retinal protonated Schiff base. Vasileiou C, Wang W, Jia X, Lee KS, Watson CT, Geiger JH, Borhan B. Proteins 77 812-822 (2009)
  3. Batrachotoxin, pyrethroids, and BTG 502 share overlapping binding sites on insect sodium channels. Du Y, Garden D, Khambay B, Zhorov BS, Dong K. Mol Pharmacol 80 426-433 (2011)


Reviews citing this publication (1)

  1. The photochemical determinants of color vision: revealing how opsins tune their chromophore's absorption wavelength. Wang W, Geiger JH, Borhan B. Bioessays 36 65-74 (2014)

Articles citing this publication (7)

  1. Arylfluorosulfates Inactivate Intracellular Lipid Binding Protein(s) through Chemoselective SuFEx Reaction with a Binding Site Tyr Residue. Chen W, Dong J, Plate L, Mortenson DE, Brighty GJ, Li S, Liu Y, Galmozzi A, Lee PS, Hulce JJ, Cravatt BF, Saez E, Powers ET, Wilson IA, Sharpless KB, Kelly JW. J Am Chem Soc 138 7353-7364 (2016)
  2. "Turn-on" protein fluorescence: in situ formation of cyanine dyes. Yapici I, Lee KS, Berbasova T, Nosrati M, Jia X, Vasileiou C, Wang W, Santos EM, Geiger JH, Borhan B. J Am Chem Soc 137 1073-1080 (2015)
  3. A Photoisomerizing Rhodopsin Mimic Observed at Atomic Resolution. Nosrati M, Berbasova T, Vasileiou C, Borhan B, Geiger JH. J Am Chem Soc 138 8802-8808 (2016)
  4. Light-Activated Reversible Imine Isomerization: Towards a Photochromic Protein Switch. Berbasova T, Santos EM, Nosrati M, Vasileiou C, Geiger JH, Borhan B. Chembiochem 17 407-414 (2016)
  5. A Genetically Encoded Ratiometric pH Probe: Wavelength Regulation-Inspired Design of pH Indicators. Berbasova T, Tahmasebi Nick S, Nosrati M, Nossoni Z, Santos EM, Vasileiou C, Geiger JH, Borhan B. Chembiochem 19 1288-1295 (2018)
  6. Engineering of a Red Fluorogenic Protein/Merocyanine Complex for Live-Cell Imaging. Santos EM, Berbasova T, Wang W, Salmani RE, Sheng W, Vasileiou C, Geiger JH, Borhan B. Chembiochem 21 723-729 (2020)
  7. Free-Energy-Based Protein Design: Re-Engineering Cellular Retinoic Acid Binding Protein II Assisted by the Moveable-Type Approach. Zhong HA, Santos EM, Vasileiou C, Zheng Z, Geiger JH, Borhan B, Merz KM. J Am Chem Soc 140 3483-3486 (2018)


Quick links