3i0x

X-ray diffraction
1.8Å resolution

Crystal structure of Clostridium acetobutylicum 8-oxoguanine glycosylase/lyase in complex with dsDNA containing adenine opposite to 8-oxoG

Released:

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ENDO3c domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 34.35 KDa
Source organism: Clostridium acetobutylicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97FM4 (Residues: 1-291; Coverage: 100%)
Gene name: CA_C2707
Sequence domains:
Structure domains:
5'-D(*AP*TP*CP*CP*AP*(8OG)P*GP*TP*CP*TP*AP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.93 KDa
Source organism: Clostridium acetobutylicum
Expression system: Not provided
5'-D(*GP*GP*TP*AP*GP*AP*CP*AP*TP*GP*GP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.75 KDa
Source organism: Clostridium acetobutylicum
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P6522
Unit cell:
a: 92.13Å b: 92.13Å c: 190.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.187 0.21
Expression systems:
  • Escherichia coli
  • Not provided