X-ray diffraction
2.27Å resolution

Crystal Structure of catalytic fragment of E. coli AlaRS in complex with AMPPCP, Ala-AMP and PCP


Function and Biology Details

Reaction catalysed:
ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Alanine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 49.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P00957 (Residues: 2-442; Coverage: 50%)
Gene names: JW2667, alaS, b2697, lovB
Structure domains: Bira Bifunctional Protein; Domain 2

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 44.355Å b: 108.745Å c: 118.985Å
α: 90° β: 90° γ: 90°
R R work R free
0.183 0.181 0.227
Expression system: Escherichia coli