3hun

X-ray diffraction
2Å resolution

Crystal structure of Penicillin binding protein 4 from Staphylococcus aureus COL in complex with Ampicillin

Released:

Function and Biology Details

Reaction catalysed:
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-alanyl-D-alanine carboxypeptidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 453 amino acids
Theoretical weight: 50.68 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2WY27 (Residues: 1-431; Coverage: 100%)
Gene names: SACOL0699, pbp4
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 90.81Å b: 98.48Å c: 99.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.251
Expression system: Escherichia coli