3htl

X-ray diffraction
1.8Å resolution

Structure of the Corynebacterium diphtheriae major pilin SpaA points to a modular pilus assembly with stabilizing isopeptide bonds

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative surface-anchored fimbrial subunit Chain: X
Molecule details ›
Chain: X
Length: 436 amino acids
Theoretical weight: 47.07 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6NF81 (Residues: 53-486; Coverage: 88%)
Gene name: DIP2013
Sequence domains: Prealbumin-like fold domain
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 34.78Å b: 64.037Å c: 199.192Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.242
Expression system: Escherichia coli BL21(DE3)