3hqh

X-ray diffraction
2.3Å resolution

Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPMATHx-MacroH2ASBCpep1

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Speckle-type POZ protein Chain: A
Molecule details ›
Chain: A
Length: 145 amino acids
Theoretical weight: 16.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43791 (Residues: 28-166; Coverage: 37%)
Gene name: SPOP
Sequence domains: MATH domain
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Core histone macro-H2A.1 Chain: M
Molecule details ›
Chain: M
Length: 15 amino acids
Theoretical weight: 1.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O75367 (Residues: 167-181; Coverage: 4%)
Gene names: H2AFY, MACROH2A1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P6522
Unit cell:
a: 44.7Å b: 44.7Å c: 268Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.257 0.238 0.276
Expression systems:
  • Escherichia coli
  • Not provided