› 3hp1

X-ray diffraction
2.31Å resolution

Crystal structure of human dCK R104M/D133A in complex with L-dT and ADP

Released:
DOI: 10.2210/pdb3hp1/pdb
PDB entry 3hp1 coloured by chain, front view.
PDB entry 3hp1 coloured by chain, side view.
PDB entry 3hp1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Extending thymidine kinase activity to the catalytic repertoire of human deoxycytidine kinase.
Hazra S, Sabini E, Ort S, Konrad M, Lavie A
Biochemistry 48 1256-63 (2009)
PMID: 19159229

Function and Biology Details

Reactions catalysed: 
ATP + deoxyguanosine = ADP + dGMP
NTP + deoxycytidine = NDP + dCMP
ATP + deoxyadenosine = ADP + dAMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxycytidine kinase Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 32.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27707 (Residues: 1-260; Coverage: 100%)
Gene name: DCK
Sequence domains: Deoxynucleoside kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand LLT 1 x LLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P43212
Unit cell:
a: 79.78Å b: 79.78Å c: 93.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.205 0.261
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Prodrug applications for targeted cancer therapy.
Giang et al. (2014)
3 more

2 mentions without citation

Structural and kinetic characterization of human deoxycytidine kinase variants able to phosphorylate 5-substituted deoxycytidine and thymidine analogues .
Hazra et al. (2010)
1 more