3hot

X-ray diffraction
3.25Å resolution

Crystal structure of the Mos1 mariner paired end complex with Mn

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mariner Mos1 transposase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 40.87 KDa
Source organism: Drosophila mauritiana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7JQ07 (Residues: 1-345; Coverage: 100%)
Gene name: mariner\T
Sequence domains: Transposase (partial DDE domain)
Structure domains:
Mos1 NTS inverted repeat DNA Chains: C, E, G
Molecule details ›
Chains: C, E, G
Length: 25 nucleotides
Theoretical weight: 7.88 KDa
Source organism: synthetic construct
Expression system: Not provided
Mos1 TS inverted repeat DNA Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 28 nucleotides
Theoretical weight: 8.53 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 120.294Å b: 84.989Å c: 132.116Å
α: 90° β: 99.21° γ: 90°
R-values:
R R work R free
0.251 0.248 0.304
Expression systems:
  • Escherichia coli
  • Not provided