› 3hot

X-ray diffraction
3.25Å resolution

Crystal structure of the Mos1 mariner paired end complex with Mn

Released:
DOI: 10.2210/pdb3hot/pdb
PDB 3hot coloured by chain and viewed from the front.
PDB 3hot coloured by chain and viewed from the side.
PDB 3hot coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Molecular architecture of the Mos1 paired-end complex: the structural basis of DNA transposition in a eukaryote.
Richardson JM, Colloms SD, Finnegan DJ, Walkinshaw MD
Cell 138 1096-108 (2009)
PMID: 19766564

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mariner Mos1 transposase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 40.87 KDa
Source organism: Drosophila mauritiana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7JQ07 (Residues: 1-345; Coverage: 100%)
Gene name: mariner\T
Sequence domains: Transposase (partial DDE domain)
Structure domains:
Mos1 NTS inverted repeat DNA Chains: C, E, G
Molecule details ›
Chains: C, E, G
Length: 25 nucleotides
Theoretical weight: 7.88 KDa
Source organism: synthetic construct
Expression system: Not provided
Mos1 TS inverted repeat DNA Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 28 nucleotides
Theoretical weight: 8.53 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand SO4 2 x SO4
1 modified residue:
Ligand 5IU 3 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 120.294Å b: 84.989Å c: 132.116Å
α: 90° β: 99.21° γ: 90°
R-values:
R R work R free
0.251 0.248 0.304
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

20 review citations

Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition.
Ochmann et al. (2021)
19 more

5 mentions without citation

Moving DNA around: DNA transposition and retroviral integration.
Montaño et al. (2011)
4 more