3hkv

X-ray diffraction
2.1Å resolution

Human poly(ADP-ribose) polymerase 10, catalytic fragment in complex with an inhibitor 3-aminobenzamide

Released:
Source organism: Homo sapiens
Entry authors: Karlberg T, Moche M, Arrowsmith CH, Berglund H, Bountra C, Collins R, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson A, Johansson I, Kotenyova T, Kotzsch A, Nielsen TK, Nordlund P, Nyman T, Persson C, Roos AK, Sagemark J, Schutz P, Siponen MI, Thorsell AG, Tresaugues L, Van Den Berg S, Weigelt J, Welin M, Wisniewska M, Schuler H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein mono-ADP-ribosyltransferase PARP10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 24.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53GL7 (Residues: 809-1017; Coverage: 20%)
Gene name: PARP10
Sequence domains: Poly(ADP-ribose) polymerase catalytic domain
Structure domains: Phosphoenolpyruvate Carboxykinase; domain 3

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P41212
Unit cell:
a: 97.38Å b: 97.38Å c: 121.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.242
Expression system: Escherichia coli