3hg7

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF D-isomer specific 2-hydroxyacid dehydrogenase family protein from Aeromonas salmonicida subsp. salmonicida A449

Released:
Entry authors: Malashkevich VN, Toro R, Morano C, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-Hacid_dh_C domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 36.5 KDa
Source organism: Aeromonas salmonicida subsp. salmonicida A449
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4SIK7 (Residues: 2-314; Coverage: 100%)
Gene name: ASA_0563
Sequence domains: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 117.055Å b: 117.055Å c: 64.336Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.264
Expression system: Escherichia coli BL21(DE3)