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Crystal structure of Thermus thermophilus Phenylalanyl-tRNA synthetase complexed with m-tyrosine

X-ray diffraction
2.9Å resolution
PDB entry 3hfz coloured by chain, front view.
PDB entry 3hfz coloured by chain, side view.
PDB entry 3hfz coloured by chain, top view.
Source organism: Thermus thermophilus
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Phenylalanine--tRNA ligase
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Multimeric state: hetero tetramer
Accessible surface area: 79537.24 Å2
Buried surface area: 26570.36 Å2
Dissociation area: 4,985.98 Å2
Dissociation energy (ΔGdiss): 46.98 kcal/mol
Dissociation entropy (TΔSdiss): 17.48 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-177825

Macromolecules

Phenylalanine--tRNA ligase alpha subunit
Chain: A
Length: 350 amino acids
Theoretical weight: 39.31 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SGX2 (Residues: 1-350; Coverage: 100%)
Gene names: TTHA1958, pheS
Pfam:
InterPro:
CATH: Bira Bifunctional Protein; Domain 2
Phenylalanine--tRNA ligase alpha subunit in PDB entry 3hfz, assembly 1, front view.
Phenylalanine--tRNA ligase alpha subunit in PDB entry 3hfz, assembly 1, side view.
Phenylalanine--tRNA ligase alpha subunit in PDB entry 3hfz, assembly 1, top view.
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Phenylalanine--tRNA ligase beta subunit
Chain: B
Length: 785 amino acids
Theoretical weight: 86.72 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SGX1 (Residues: 1-785; Coverage: 100%)
Gene names: TTHA1959, pheT
Pfam:
InterPro:
CATH:
Phenylalanine--tRNA ligase beta subunit in PDB entry 3hfz, assembly 1, front view.
Phenylalanine--tRNA ligase beta subunit in PDB entry 3hfz, assembly 1, side view.
Phenylalanine--tRNA ligase beta subunit in PDB entry 3hfz, assembly 1, top view.
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