› 3hfu

X-ray diffraction
2.6Å resolution

Crystal structure of the ligand binding domain of E. coli CynR with its specific effector azide

Released:
DOI: 10.2210/pdb3hfu/pdb
PDB entry 3hfu coloured by chain, front view.
PDB entry 3hfu coloured by chain, side view.
PDB entry 3hfu coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Singer AU, Evdokimova E, Kagan O, Dong A, Edwards AM, Savchenko A

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator CynR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 238 amino acids
Theoretical weight: 26.41 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P27111 (Residues: 63-299; Coverage: 79%)
Gene names: JW5894, b0338, cynR
Sequence domains: LysR substrate binding domain
Structure domains: D-Maltodextrin-Binding Protein; domain 2

Ligands and Environments

2 bound ligands:
Ligand AZI 4 x AZI
Ligand EDO 2 x EDO
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 62.176Å b: 98.363Å c: 86.272Å
α: 90° β: 103.769° γ: 90°
R-values:
R R work R free
0.226 0.223 0.274
Expression system: Escherichia coli

Quick links