X-ray diffraction
1.3Å resolution

Crystal structure of E. coli HPPK(N10A) in complex with MgAMPCPP

Source organism: Escherichia coli K-12
Entry authors: Blaszczyk J, Li Y, Yan H, Ji X

Function and Biology Details

Reaction catalysed:
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 17.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P26281 (Residues: 2-159; Coverage: 99%)
Gene names: JW0138, b0142, folK
Sequence domains: 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK)
Structure domains: 7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: C2
Unit cell:
a: 79.83Å b: 53.02Å c: 36.6Å
α: 90° β: 102.57° γ: 90°
R R work R free
0.168 0.167 0.22
Expression system: Escherichia coli