› 3h8i

X-ray diffraction
2.65Å resolution

The first X-ray structure of a sulfide:quinone oxidoreductase: Insights into sulfide oxidation mechanism

Released:
DOI: 10.2210/pdb3h8i/pdb
PDB entry 3h8i coloured by chain, front view.
PDB entry 3h8i coloured by chain, side view.
PDB entry 3h8i coloured by chain, top view.
Source organism: Acidianus ambivalens
Primary publication:
Structural and functional insights into sulfide:quinone oxidoreductase.
Brito JA, Sousa FL, Stelter M, Bandeiras TM, Vonrhein C, Teixeira M, Pereira MM, Archer M
Biochemistry 48 5613-22 (2009)
PMID: 19438211

Function and Biology Details

Reaction catalysed: 
n sulfide + n quinone = polysulfide + n quinol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfide-quinone reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 409 amino acids
Theoretical weight: 45.17 KDa
Source organism: Acidianus ambivalens
UniProt:
  • Canonical: Q7ZAG8 (Residues: 1-409; Coverage: 100%)
Gene names: noxA, sqr
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand S3H 2 x S3H
Ligand PO4 9 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6522
Unit cell:
a: 178.55Å b: 178.55Å c: 162.31Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.196 0.225

Quick links

Citations

13 review citations

Enzymology of H2S biogenesis, decay and signaling.
Kabil et al. (2014)
12 more

1 mention without citation

Novel base-pairing interactions at the tRNA wobble position crucial for accurate reading of the genetic code.
Rozov et al. (2016)