3h8e

X-ray diffraction
2.75Å resolution

Low pH native structure of leucine aminopeptidase from Pseudomonas putida

Released:

Function and Biology Details

Reactions catalysed:
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosol aminopeptidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 497 amino acids
Theoretical weight: 52.53 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: O86436 (Residues: 1-497; Coverage: 100%)
Gene name: pepA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P63
Unit cell:
a: 116.946Å b: 116.946Å c: 137.899Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.267
Expression system: Escherichia coli