3h7i

X-ray diffraction
1.5Å resolution

Structure of the metal-free D132N T4 RNase H

Released:
Source organism: Escherichia virus T4
Entry authors: Tomanicek SJ, Mueser TC

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease H Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 35.61 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P13319 (Residues: 1-305; Coverage: 100%)
Gene names: 33.2, das, rnh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 36.011Å b: 85.858Å c: 57.26Å
α: 90° β: 92° γ: 90°
R-values:
R R work R free
0.181 0.18 0.213
Expression system: Escherichia coli