3h6i

X-ray diffraction
2.43Å resolution

Crystal Structure of Mycobacterium Tuberculosis Proteasome Modified by inhibitor GL1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only 28-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proteasome subunit alpha Chains: 1, A, B, D, F, I, K, M, O, Q, S, U, W, Y
Molecule details ›
Chains: 1, A, B, D, F, I, K, M, O, Q, S, U, W, Y
Length: 248 amino acids
Theoretical weight: 26.91 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHU1 (Residues: 1-248; Coverage: 100%)
Gene names: Rv2109c, prcA
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta Chains: 2, C, E, G, H, J, L, N, P, R, T, V, X, Z
Molecule details ›
Chains: 2, C, E, G, H, J, L, N, P, R, T, V, X, Z
Length: 240 amino acids
Theoretical weight: 25.3 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHT9 (Residues: 59-291; Coverage: 80%)
Gene names: Rv2110c, prcB
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 171.615Å b: 117.667Å c: 196.335Å
α: 90° β: 113.65° γ: 90°
R-values:
R R work R free
0.204 0.204 0.232
Expression system: Escherichia coli