› 3h4t

X-ray diffraction
1.15Å resolution

Chimeric Glycosyltransferase for the generation of novel natural products - GtfAH1 in complex with UDP-2F-Glc

Released:
DOI: 10.2210/pdb3h4t/pdb
PDB entry 3h4t coloured by chain, front view.
PDB entry 3h4t coloured by chain, side view.
PDB entry 3h4t coloured by chain, top view.
Source organisms:
Primary publication:
Chimeric glycosyltransferases for the generation of hybrid glycopeptides.
Truman AW, Dias MV, Wu S, Blundell TL, Huang F, Spencer JB
Chem Biol 16 676-85 (2009)
PMID: 19549605

Function and Biology Details

Reaction catalysed: 
dTDP-beta-L-4-epi-vancosamine + devancoaminyl-vancomycin = dTDP + chloroorienticin B
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161221 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GtfA protein; dTDP-epi-vancosaminyltransferase Chain: A
Molecule details ›
Chain: A
Length: 404 amino acids
Theoretical weight: 42.11 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96558 (Residues: 1-205; Coverage: 52%)
  • Canonical: Q6ZZJ7 (Residues: 209-393; Coverage: 47%)
Gene names: FHX34_1062, gtfA, tcp8
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand PO4 4 x PO4
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 111.107Å b: 129.693Å c: 67.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Enzymatic methods for glyco(diversification/randomization) of drugs and small molecules.
Gantt et al. (2011)
14 more

1 mention without citation

Comparative interactions of withanolides and sterols with two members of sterol glycosyltransferases from Withania somnifera.
Pandey et al. (2015)