› 3h3p

X-ray diffraction
2.7Å resolution

Crystal structure of HIV epitope-scaffold 4E10 Fv complex

Released:
DOI: 10.2210/pdb3h3p/pdb
PDB 3h3p coloured by chain and viewed from the front.
PDB 3h3p coloured by chain and viewed from the side.
PDB 3h3p coloured by chain and viewed from the top.
Source organisms:
Entry author: Holmes MA

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fv 4E10 heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 137 amino acids
Theoretical weight: 14.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fv 4E10 light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 114 amino acids
Theoretical weight: 12.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
4E10_S0_1TJLC_004_N Chains: S, T
Molecule details ›
Chains: S, T
Length: 85 amino acids
Theoretical weight: 10.5 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6522
Unit cell:
a: 92.4Å b: 92.4Å c: 272.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.229 0.287
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Advances in structure-based vaccine design.
Kulp et al. (2013)
6 more

2 mentions without citation

Interactions between lipids and human anti-HIV antibody 4E10 can be reduced without ablating neutralizing activity.
Xu et al. (2010)
1 more