› 3h20

X-ray diffraction
1.99Å resolution

Crystal structure of primase RepB'

Released:
DOI: 10.2210/pdb3h20/pdb
PDB 3h20 coloured by chain and viewed from the front.
PDB 3h20 coloured by chain and viewed from the side.
PDB 3h20 coloured by chain and viewed from the top.
Source organism: Plasmid RSF1010
Primary publication:
Structure and function of primase RepB' encoded by broad-host-range plasmid RSF1010 that replicates exclusively in leading-strand mode.
Geibel S, Banchenko S, Engel M, Lanka E, Saenger W
Proc. Natl. Acad. Sci. U.S.A. 106 7810-5 (2009)
PMID: 19416864

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RepB-like DNA primase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.96 KDa
Source organism: Plasmid RSF1010
Expression system: Escherichia coli
UniProt:
  • Canonical: Q52349 (Residues: 1-323; Coverage: 100%)
Gene name: repB
Sequence domains: RepB DNA-primase from phage plasmid
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand DPO 1 x DPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P43212
Unit cell:
a: 91.74Å b: 91.74Å c: 83.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.232
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structures to complement the archaeo-eukaryotic primases catalytic cycle description: What's next?
Boudet et al. (2015)
4 more

4 mentions without citation

Initiating DNA replication: a matter of prime importance.
Bell SD. (2019)
3 more