3gyq

X-ray diffraction
2.45Å resolution

Structure of the Thiostrepton-Resistance Methyltransferase S-adenosyl-L-methionine Complex

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + adenosine(1067) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyladenosine(1067) in 23S rRNA
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
23S rRNA (adenosine(1067)-2'-O)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 272 amino acids
Theoretical weight: 29.22 KDa
Source organism: Streptomyces cyaneus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18644 (Residues: 1-269; Coverage: 100%)
Gene names: tsnR, tsr
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 40.96Å b: 55.77Å c: 215.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.268
Expression system: Escherichia coli