› 3gts

X-ray diffraction
2.2Å resolution

Crystal Structure of Dicamba Monooxygenase with Non-heme Iron and Dicamba

Released:
DOI: 10.2210/pdb3gts/pdb
PDB entry 3gts coloured by chain, front view.
PDB entry 3gts coloured by chain, side view.
PDB entry 3gts coloured by chain, top view.
Source organism: Stenotrophomonas maltophilia
Primary publication:
Dicamba monooxygenase: structural insights into a dynamic Rieske oxygenase that catalyzes an exocyclic monooxygenation.
D'Ordine RL, Rydel TJ, Storek MJ, Sturman EJ, Moshiri F, Bartlett RK, Brown GR, Eilers RJ, Dart C, Qi Y, Flasinski S, Franklin SJ
J Mol Biol 392 481-97 (2009)
PMID: 19616009

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177801 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dicamba O-demethylase, oxygenase component Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 349 amino acids
Theoretical weight: 38.64 KDa
Source organism: Stenotrophomonas maltophilia
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5S3I3 (Residues: 1-339, 2-339; Coverage: 100%)
Gene name: ddmC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand FES 3 x FES
Ligand FE 2 x FE
Ligand D3M 2 x D3M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P32
Unit cell:
a: 80.8Å b: 80.8Å c: 159.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.23 0.266
Expression system: Escherichia coli

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Citations

3 review citations

Dioxygen activation by nonheme iron enzymes with the 2-His-1-carboxylate facial triad that generate high-valent oxoiron oxidants.
Kal et al. (2017)
2 more

2 mentions without citation

Molecular genetic analysis of the glycosyltransferase Fringe in Drosophila.
Correia et al. (2003)
1 more