3gs2

X-ray diffraction
1.7Å resolution

Ring1B C-terminal domain/Cbx7 Cbox Complex

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase RING2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 111 amino acids
Theoretical weight: 12.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99496 (Residues: 223-333; Coverage: 33%)
Gene names: BAP1, DING, HIPI3, RING1B, RNF2
Sequence domains: RAWUL domain RING finger- and WD40-associated ubiquitin-like
Structure domains: Ubiquitin-like (UB roll)
Chromobox protein homolog 7 Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95931 (Residues: 219-248; Coverage: 12%)
Gene name: CBX7

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 44.762Å b: 50.547Å c: 124.074Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.243
Expression system: Escherichia coli