› 3goi

X-ray diffraction
2.52Å resolution

Human glucokinase in complex with a synthetic activator

Released:
DOI: 10.2210/pdb3goi/pdb
PDB entry 3goi coloured by chain, front view.
PDB entry 3goi coloured by chain, side view.
PDB entry 3goi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery of novel 3,6-disubstituted 2-pyridinecarboxamide derivatives as GK activators.
Mitsuya M, Kamata K, Bamba M, Watanabe H, Sasaki Y, Sasaki K, Ohyama S, Hosaka H, Nagata Y, Eiki J, Nishimura T
Bioorg Med Chem Lett 19 2718-21 (2009)
PMID: 19362831

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-4 Chain: A
Molecule details ›
Chain: A
Length: 455 amino acids
Theoretical weight: 50.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35557 (Residues: 16-465; Coverage: 97%)
Gene name: GCK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GLC 1 x GLC
Ligand LOI 1 x LOI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P6522
Unit cell:
a: 80.085Å b: 80.085Å c: 323.974Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.205 0.282
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Homotropic allosteric regulation in monomeric mammalian glucokinase.
Larion et al. (2012)
1 more

2 mentions without citation

Strain analysis of protein structures and low dimensionality of mechanical allosteric couplings.
Mitchell et al. (2016)
1 more