X-ray diffraction
1.7Å resolution

Crystal structure of the Salmonella typhimurium FadA 3-ketoacyl-CoA thiolase

Entry authors: Anderson SM, Skarina T, Onopriyenko O, Wawrzak Z, Papazisi L, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
3-ketoacyl-CoA thiolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 41.99 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
  • Canonical: P0A2H7 (Residues: 1-387; Coverage: 100%)
Gene names: STM3982, STMD1.7, fadA
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 73Å b: 64.5Å c: 74.4Å
α: 90° β: 104.5° γ: 90°
R R work R free
0.145 0.142 0.192
Expression system: Escherichia coli